FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_NFKB2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_NFKB2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9113
Sequences flagged as poor quality0
Sequence length300
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA819989.97037199604961No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA2082.282453637660485No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGCAGCTTCTTAA360.39504005267200704No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGCAGCTTCTTAA340.3730933830791177No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGCAGCTTCTTAA330.3621200482826731No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTAA280.3072533743004499No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGTTCTTAA210.23044003072533745No Hit
CGATCGATACAGGTATTCTTGTGGAAAGACGAAACACCGCAGCTTCTTAA180.19752002633600352No Hit
CGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGGGCTTCCTTG160.17557335674311425No Hit
CGATCGATACAGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA160.17557335674311425No Hit
CGATCGATACAGGTATTTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA150.1646000219466696No Hit
CGATCGATACGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA140.15362668715022496No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAGCTTCTTAA140.15362668715022496No Hit
CGATCGATAAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA130.14265335235378032No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTTTAA120.1316800175573357No Hit
CGATCGATACAGGAATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTA120.1316800175573357No Hit
CGATCGATACAGGTATTCTTTGGAAAGGACGAAACACCGCAGCTTCTTAA110.12070668276089104No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA110.12070668276089104No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTCTTAA100.10973334796444639No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)