FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_NFKB2_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_NFKB2_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9916
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCA912592.02299314239613No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACTATCCCAC500.5042355788624445No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGGACCTATCCCAC390.39330375151270675No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCAC390.39330375151270675No Hit
GATCGATAACGCATTTCTGTGGAAAGGACGAAACACCGGACCTATCCCAC300.30254134731746674No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCAA270.27228721258572003No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGACCTATCCCAC210.21177894312222673No Hit
GATCGATAACGCATTTCTTGTGAAAGGACGAAACACCGGACCTATCCCAC180.18152480839048005No Hit
GATCGATAACGCATTTTTGTGGAAAGGACGAAACACCGGACCTATCCCAC160.16135538523598225No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGCAGCTTCTTAA140.14118596208148448No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAACACCGGACCTATCCCAC130.1311012505042356No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCC130.1311012505042356No Hit
GATCGATAACGCATTTATTGTGGAAAGGACGAAACACCGGACCTATCCCA120.12101653892698667No Hit
GATCGATAACGCATTCTTGTGGAAAGGACGAAACACCGGACCTATCCCAC120.12101653892698667No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGGACCTATCCCAC120.12101653892698667No Hit
GATCGATAACGATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCAC110.11093182734973779No Hit
GATCGATAACGCATTTCTTGTGGAAAGACGAAACACCGGACCTATCCCAC110.11093182734973779No Hit
GATCGATAACGCATTTCTTTGGAAAGGACGAAACACCGGACCTATCCCAC100.10084711577248891No Hit
GATCGATACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTATCCCAC100.10084711577248891No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGGACCTTCCCAC100.10084711577248891No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)