FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_MAP3K14_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_MAP3K14_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7159
Sequences flagged as poor quality0
Sequence length300
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAG666393.07165805280067No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGCTGTGTAGACAGC360.5028635284257578No Hit
ATCGATTCCTTGGTTCTTGTGAAAGGACGAAACACCGCTGTGTAGACAGC240.33524235228383853No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC230.32127392093867857No Hit
ATCGATTCCGTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAG120.16762117614191926No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGACGAAACACCGCTGTGTAGACAGC110.15365274479675933No Hit
ATCGATTCCTTGGTTCTTGTAGAAAGGACGAAACACCGCTGTGTAGACAG100.13968431345159937No Hit
ATCGATTCCTTGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC90.12571588210643944No Hit
ATCGATTCCTTGGTTTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC90.12571588210643944No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACGCTGTGTAGACAGC80.11174745076127951No Hit
ATCGATTCCTGGTTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC80.11174745076127951No Hit
ATCGATTCCTTGGTCTTGTGGAAAGGACGAAACACCGCTGTGTAGACAGC80.11174745076127951No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)