FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_MAP3K14_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_MAP3K14_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9591
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA871690.87686372641018No Hit
CGATCATGATAGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA981.0217912626420602No Hit
CGATCATGATCGTATTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA910.9488061724533416No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAGCCAAGGAGAA570.59430716296528No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAAAGCCAAGGAGAA310.32321968512146804No Hit
CGATCATGATCGTCTTGTGGAAGGACGAAACACCGCAAAGCCAAGGAGAA300.31279324366593686No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA290.30236680221040557No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACGCAAAGCCAAGGAGAA290.30236680221040557No Hit
CGATCATGATCGTCTTGTGAAAGGACGAAACACCGCAAAGCCAAGGAGAA280.29194036075487434No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAGGAGAA270.28151391929934316No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCAAGGAGAA180.1876759461995621No Hit
CGATCATGAGCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGA170.17724950474403087No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGGAA170.17724950474403087No Hit
CGATCATGATCGTCTTGGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA170.17724950474403087No Hit
CGATCATGATGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA160.16682306328849963No Hit
CGATCATGATCGTCTTTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA160.16682306328849963No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGCAAAGCCAAGAGAA140.14597018037743717No Hit
CGATCATGATCGTTTGTGGAAAGGACGAAACACCGCAAAGCCAAGGAGAA110.1146908560108435No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)