FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_LIGHT_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_LIGHT_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12683
Sequences flagged as poor quality0
Sequence length300
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC1156191.15351257588898No Hit
CGATCGATACCGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTC2932.3101789797366554No Hit
CGATCGATACAGGTATTCTTGTGGAAGGACGAAACACCGGAGCAATTTCG510.40211306473231884No Hit
CGATCGATACAGGTATTCTGTGGAAAGGACGAAACACCGGAGCAATTTCG340.26807537648821256No Hit
CGATCGATACAGGTATTCTTGTGGAAAGACGAAACACCGGAGCAATTTCG250.19711424741780337No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGGAGCAATTTCG240.18922967752109124No Hit
CGATCGATACGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG220.17346053772766695No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTCG180.1419222581408184No Hit
CGATCGATACAGGTATCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG180.1419222581408184No Hit
CGATCGATAAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG170.13403768824410628No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGAATTTCG160.12615311834739415No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCATTTCG150.11826854845068202No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAACACCGGAGCAATTTCG130.10249940865725775No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACAAAACACCGGAGCAATTTC130.10249940865725775No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)