FastQCFastQC Report
Tue 14 Sep 2021
000000000-G67TC_l01_n01_LTBR_LIGHT_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G67TC_l01_n01_LTBR_LIGHT_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10397
Sequences flagged as poor quality0
Sequence length300
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAA952891.64181975569876No Hit
GATCGATAACGCATTTCTTGTGGAAGGACGAAACACCGCAAACAAGCAAC620.5963258632297779No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAACAAGCAAC540.5193805905549678No Hit
GATCGATAACGCATTTCTGTGGAAAGGACGAAACACCGCAAACAAGCAAC350.33663556795229393No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAGCAAC320.30778109069924015No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAAACAAGCAAC300.2885447725305377No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGGAGCAATTTCG230.2212176589400789No Hit
GATCGATAACGCATTTCTTGTGAAAGGACGAAACACCGCAAACAAGCAAC220.21159949985572762No Hit
GATCGATAACGCATTTTTGTGGAAAGGACGAAACACCGCAAACAAGCAAC190.18274502260267386No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACGCAAACAAGCAAC170.16350870443397134No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAC170.16350870443397134No Hit
GATCGATAACGCATTTCTTGGGAAAGGACGAAACACCGCAAACAAGCAAC160.15389054534962007No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAACACCGCAAACAAGCAAC150.14427238626526884No Hit
GATCGATAACGCATTTCTTTGGAAAGGACGAAACACCGCAAACAAGCAAC140.13465422718091757No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGAAC140.13465422718091757No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGCAAAAAGCAAC130.12503606809656634No Hit
GATCGATAACGCATTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAAC120.11541790901221506No Hit
GATCGATAACGCATTTCTTGTGGAAAGACGAAACACCGCAAACAAGCAAC120.11541790901221506No Hit
GATCGATAACGATTTCTTGTGGAAAGGACGAAACACCGCAAACAAGCAAC110.10579974992786381No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)