FastQCFastQC Report
Fri 10 Sep 2021
000000000-G64RH_l01_n02_WGS19-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G64RH_l01_n02_WGS19-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77715
Sequences flagged as poor quality0
Sequence length287
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT3534145.47513350061121No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT2309529.71755774303545No Hit
ACTACTTAGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT18512.3817795792318086No Hit
TGCAACACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT16992.1861931416071543No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGCG8251.0615711252653928No Hit
CCAGAGCGTCGCTGTCCGTGTGCTCCCGCAGGATTCGCAGGTGCGTGGAG5940.7643312101910829No Hit
GTCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT3670.4722383066332111No Hit
GTACTGATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT3100.3988933925239658No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGCGTACGATTCTTTGATTA2920.3757318406999936No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGAGCG2520.3242617255356109No Hit
GGCGATATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT2270.2920929035578717No Hit
TAAGCGAGGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT2060.2650710930965708No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG2040.2624975873383517No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGAC1920.24705655278903688No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACA1830.23547577687705076No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGATG1750.22518175384417422No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTGAGAT1630.2097407192948594No Hit
GGCGATACGACAGCAGTATCTGCTATGGGACAGCAGTATCATCGACTATG1610.2071672135366403No Hit
GTTTGCATGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1540.1981599433828733No Hit
TGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1460.1878659203499968No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGTATGGGCGAGCGCTATGAGC1400.18014540307533938No Hit
CCGCTATCGACAGCAGTATCTGCTGTCACTATGGGAGAGCGCGCTCGTCG1380.17757189731712025No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATCTATGGGCGAGCGCTATGAGC1320.16985138004246286No Hit
CCGCTATCGACAGCAGTACTATGGGCGAGCGCTCTATGGGAGAGCGCGCT1310.16856462716335327No Hit
GGCGATACGACAGCAGTATCTGTTGTCATGGGACTGCACGACAGTCGACT1300.1672778742842437No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTACACGACAGTCGACT1250.16084410988869588No Hit
GGCTATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGACT1230.15827060413047675No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCGAGC1160.14926333397670977No Hit
GGCGATACGACAGCAGTATCTGCTGTCATACTATGGGCGAGCGCTATGAG1160.14926333397670977No Hit
CAAAGGCGCAAAAATTATGCGAAATAAAGCCCGAAAACGAAACGGAGGCA1030.1325355465482854No Hit
CCGCTATCGACAGCAGTATCTGCTGTCATGGGACTGCACGACTATGGGTC910.1170945119989706No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTCTATGGGCGAGCTAT880.1132342533616419No Hit
GGCGATACGACAGCAGTATCTGCTGTCATGGGACTGCACGACAGTCTATG830.10680048896609406No Hit
CCGCTATCGACAGCAGTATCTGCTGCTATGGGAGAGCGCGCTCGTCGACT780.10036672457054623No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)