FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245348
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG28171.1481650553499518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTG207.8702215E-8280.998573
TCGTGCA158.78295E-6280.998575
TATCATC9300.0273.444859
AGTATCA9950.0269.702157
CAGCCTA252.3946995E-7224.798873
GGATCGT252.3946995E-7224.798872
ACAGCCT252.3946995E-7224.798872
CGTGCAA202.7679636E-5210.748936
TCACGTA202.7679636E-5210.748934
GAACTCC150.0032944286187.332381
ACCTCCT401.178887E-8175.62412
TACAGCA401.178887E-8175.62411
GACCTCC401.178887E-8175.62411
CGGATCG256.738538E-5168.599151
GCAGTCT700.0160.570625
GCAGCTA351.2803212E-6160.570625
CAGTATC17250.0158.825296
GACAGCC452.3826942E-8156.11031
CAGTCTA657.2759576E-12151.306926
CCTTATC200.007788226140.499286
Produced by FastQC (version 0.11.5)