FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_WTA002-Lib4-C9G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_WTA002-Lib4-C9G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188591
Sequences flagged as poor quality0
Sequence length287
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG2792614.807705563892231No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA24071.2763069287505766No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC16400.8696067150606338No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG14610.7746923235997476No Hit
GACAGCAGTATCATCGATGATGGATGATGGACTCCATCCATCCATCAGCC13660.7243187638858695No Hit
GACAGCAGTATCATCGAGGTTTTTTGTAGAGGTTTTACTTGCTTTAAAAA13060.6925038840665779No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT10460.5546394048496481No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG10440.5535789088556717No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG9500.5037355971387818No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA9210.4883584052261243No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT9170.4862374132381715No Hit
GACAGCAGTATCATCGAAATATTTGATGTATAGTGCCTTGACTAGAGATC9040.47934418927732503No Hit
GACAGCAGTATCATACTTGTCGAGATACTTGTCGTGGATACTTGACGTAT8870.4703299733285257No Hit
GACAGCAGTATCATCGTCGTTTGTTGAATTTATTATTAGTATGTAAGTGT8150.432152117545376No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC8020.4252588935845295No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA7770.41200269365982467No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC7640.40510946969897815No Hit
GACAGCAGTACCAGTGAAATCCAAGCATTTTCTAAATTAAATGTATTCTT7510.3982162457381317No Hit
GACAGCAGTATCATCAGCAGTATGGAGTCGACAGCAGTATCTGCTGTCAT7500.3976859977411435No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG5970.31655805420195027No Hit
GACAGCAGTATCATCGACTACTGTCGACATAGCCATCGTAGCTATGGAGT5920.3139068142170093No Hit
GACAGCAGTATCATCGACCTACCACAATAACCAGTTTGTTTTGGGATTCT5890.3123160702260447No Hit
GACAGCAGTATCATCGAAACTTTTTTGTATCATTTTGAAGCATCATTTTT5840.30966483024110375No Hit
GACAGCAGTATCATCGAGAACTCTTGCTTGCTTTGCTATTTACACCACAA5440.2884549103615761No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA5370.2847431743826588No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG5290.28050119040675325No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA5260.27891044641578866No Hit
GACAGCAGTATCATCGGGCTGCAGGAATTCGATAAAAGTTTTGTTACTTT5210.27625920643084767No Hit
GACAGCAGTATCATCGATGATACCATCGATATGGAGTCGAGAGCGCGCTA5190.27519871043687133No Hit
GACAGCAGTATCATCGATGCAGTTGACGATCATCATCATCATCATCATCA5090.2698962304669894No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT5080.2693659824700012No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA5020.2661844944880721No Hit
GACAGCAGTATCATCGATCATGGATCGACAAATGGCAGTATTCATCCACA4940.26194251051216655No Hit
GACAGCAGTATCATCGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAG4910.26035176652120195No Hit
GACAGCAGTATCATCGAAGTAGATCTTTAGATACACCCTAATACTAAATA4850.2571702785392728No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC4740.25133755057240276No Hit
GACAGCAGTATCATCTTATTATTATAGTTGTTATTTTTGATATATATAAA4710.24974680658143814No Hit
GACAGCAGTAAGAGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATT4700.24921655858444994No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG4580.24285358262059165No Hit
GACAGCAGTATCATCCATCACAAATAAAGCATTTTTTTCACTGCATTCTA4550.24126283862962705No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG4440.23543011066275696No Hit
GACAGCAGTATCATCTATGATGATAAATAAATAAATTTAATAAATTAAAT4370.23171837468383963No Hit
GACAGCAGTATCATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACA4170.2211134147440758No Hit
GACAGCAGTATCATCGACTACAGAGATTTAAAGCTCTAAGATTCCAACCT4150.2200529187500994No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGGCATAGACAT3950.20944795881033557No Hit
GACAGCAGTATCATCATCATTCACCTAGTTCCAGTGAAATCCAAGCATTT3690.1956615108886426No Hit
GACAGCAGTATCATCGATACTGGATACTGGATACGATACTCCATCGTATG3650.19354051890068985No Hit
GACAGCAGTATCATCGAATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG3460.18346580695791423No Hit
GACAGCAGTATCATCAAACACTGGATGTCACTTTCAGTTCAAATTGTAAC3450.18293555896092603No Hit
GACAGCAGTATCATCGATACTTCATCGATGTCTATGCTCTCGACTCCATA3440.18240531096393783No Hit
GACAGCAGTATCATCGATGATATCGCGCAGACCGCGCTAAATGTCGATGT3440.18240531096393783No Hit
GACAGCAGTATCTCGAGTCTGGATCCTCTAGAGATCCACTAGTGTCGACG3390.17975407097899687No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA3360.1781633269880323No Hit
GACAGCAGTATCATCGATCGTAGCGACTGTAGTCGACTCCATACTCGATG3270.17339109501513858No Hit
GACAGCAGAATGCCTTTAATGAGGAAAACCTGTTTTGCTCAGAAGAAATG3270.17339109501513858No Hit
GACAGCAGTATCATCTGTCCGCGGAAGCGAAACGAAACTCACCACGCCAT3230.17127010302718582No Hit
GACAGCAGTATCATCGGAGTGACTGGATGGATACTGGATATAAATATCCA3160.1675583670482685No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA3100.16437687906633933No Hit
CTATGGAGTCGAGAGCGCACTATGGAGTCGTCAGCAGTACTACTGACGAT3080.16331638307236293No Hit
GACAGCAGTATCATCGCTATGGAGTCGCGAGCGCTATGAGCGACTATGGA3030.160665143087422No Hit
GACAGCAGTATCATCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGT3010.1596046470934456No Hit
GACAGCAGTATCATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA2990.15854415109946923No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGGAGTCGACAGAGATATCAT2870.15218117513561094No Hit
GACAGCAGTATCAAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGT2830.15006018314765818No Hit
GACAGCAGTATCATCGATGGAGTTTGGCAGACATGGACAAGGCAATGCAA2750.14581819917175262No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG2720.14422745518078806No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC2690.1426367111898235No Hit
GACAGCAGTATCATACTTGCTGTACATAGTAGCGATGTACATGCTGTATA2680.1421064631928353No Hit
GACAGCAGTATCATCGAATAGAGAGAATAACGAATAACGGCCAGAGAAAT2600.13786447921692976No Hit
GACAGCAGTATCATCGATCACCTCCCCCTGAACCTGAAACATAAAATGAA2590.13733423121994157No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG2570.1362737352259652No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGAGCGCGACCCGGGCTGCA2540.1346829912350006No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCATAGACATCGACTAGACATG2510.13309224724403604No Hit
GACAGCAGTATCATACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG2510.13309224724403604No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA2490.13203175125005964No Hit
GACAGCAGTATCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGA2440.12938051126511868No Hit
GACAGCAGTATCATCGAGTCGACATGGAGTCGTCAGCAGTACTACTGACT2390.12672927128017775No Hit
GACATTGCTAAGTTTTTTGAGTCATGCTGTGTTTAGTAATAGAACTCTTG2360.12513852728921318No Hit
GACAGCAGTATCATCGATGCATCTACACAAGGAACAAACACTGGATGTCA2330.1235477832982486No Hit
GACAGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA2330.1235477832982486No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT2330.1235477832982486No Hit
GACAGCAGTAACTATGGAGTCGAGAGCGCGCTCGTCGAAAATTATGGAAA2320.1230175353012604No Hit
GACAGCAGTATCATCGAGTCATCATGGAGTCGCGAGCGCTATGAGCTATG2300.12195703930728402No Hit
GACAGCAGTATCATCTGTCTGGAATCGAGAGCGCGCTCGTCGACTATGGA2280.12089654331330763No Hit
GACAGCAGTATCATCGAGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC2240.11877555132535486No Hit
GACAGCAGTATCATCGTTCCCCCTGAACCTGAAACATAAAATGAATGCAA2210.11718480733439028No Hit
GACAGCAGTATCAGACTGCACGACAGTCGACTATGGAGTCGCGAGCGCTA2200.11665455933740211No Hit
GAACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGACAGCAGTG2170.11506381534643753No Hit
GACAGCAGTATCATCGAGAGTCGCGAGCGCTATGAGCGCAGAGTATGGAT2160.11453356734944933No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACAGCAGTATCTGCTGTCATG2160.11453356734944933No Hit
GACCAATTCCAGTATCAAAATATAATAAAACTTAATATCTATTCAAATTA2130.11294282335848477No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCTATGGAGTCGTCAGC2120.11241257536149657No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCTATGAGCGACTATGGATC2110.11188232736450837No Hit
GACAGCAGTATAATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG2090.110821831370532No Hit
GACAGCAGTATCATCGATCGACAAATGGCAGTATTCATCCACAGGATCTT2070.10976133537655562No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGAAGTCGTCAGCAGT2070.10976133537655562No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACGATGGAGTCGACAGC2050.10870083938257923No Hit
GACAGCAGTATCGATGATCGATATAGCATACATTATACGAAGTTATGCTA2040.10817059138559106No Hit
GACAGCAGTATCATCGACCCATCGACTCCCATCGACCCGGGCTGCAGGAA2000.10604959939763828No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGACGACTATGG2000.10604959939763828No Hit
GACAGCAGTATCAGAAAGTAGCAGTAGCAGAAAGTATAGGAACTTCAGAG1970.10445885540667371No Hit
GACAGCAGTATCATCGATCTATGGAGTCTCGACCTGACCTACCACAATAA1970.10445885540667371No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC1950.10339835941269732No Hit
GACAGCAGTATCATCGAGCAGTATGGAGTCGACTACAGTCGCTACGACAT1930.10233786341872093No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCCTATGGAGTCGTCAGCAGTA1930.10233786341872093No Hit
GACAGAAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG1930.10233786341872093No Hit
GACAGCAGTATCATTGGACTCCAGTATCGACTCCAATTAAATGTATTCTT1910.10127736742474454No Hit
GACAGCAGTATCATCGAGTGCACCCGATGAGGAAAACCTGTTTTGCTCAG1890.10021687143076817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAGCA109.785389E-4281.01
TATCATC114650.0276.710859
GTATCAT123350.0272.685038
AGTATCA128800.0272.600527
GTACCAG750.0262.266668
TATCCTA4300.0248.325589
GTATCCT4550.0243.945058
ACAGCCT4300.0241.79072
CAGCCTA4350.0239.011493
AGCAGCT3050.0211.901644
TATCACT1600.0210.759
AGTATCC5400.0210.757
GTATCAA2550.0209.372548
ACATTGC550.0204.363652
GCAGCTA3100.0203.951615
ACAGCTA3700.0201.256742
TATAATC1550.0199.419369
GTATCAC2400.0199.041668
AGTACCA1350.0197.740757
GTATCTA3100.0194.88718
Produced by FastQC (version 0.11.5)