FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_WTA002-Lib3-C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_WTA002-Lib3-C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495110
Sequences flagged as poor quality0
Sequence length287
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG8876717.92874310759225No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG59621.2041768495889802No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC53461.0797600533214842No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG32480.6560158348649795No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG27940.5643190402132859No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA26060.5263476803134657No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA23600.4766617519339137No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA22220.44878915796489666No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA21980.4439417503181112No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG21860.4415180464947184No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG21150.4271777988729777No Hit
GACAGCAGTATCATCGAAATATTTGATGTATAGTGCCTTGACTAGAGATC21020.4245521197309689No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC19500.39385187130132704No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT16810.3395205105936055No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA14270.28821877966512494No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14040.2835733473369554No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA13940.2815535941507948No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC12390.2502474197653047No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT12290.24822766657914405No Hit
GACAGCAGTATCATCGACTACTGTCGACATAGCCATCGTAGCTATGGAGT11740.23711902405526042No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCTATGGAGTCGTCAGC11710.23651309809941223No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG11540.23307951768293916No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGATCGACAAATGGCAG11360.22944396194785No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC11220.22661630748722505No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC11140.22500050493829654No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT11040.2229807517521359No Hit
GACAGCAGTATCATCATGACCGTCAGTCGCGCGAGCGCGACCCGGGCTGC10950.2211629738845913No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA10850.21914322069843065No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG9890.1997535901112884No Hit
GACAGCAGTATCATCGAGCGCGCTCGTCGACTATGGAGTCGTCAGCATTG9720.1963200096948153No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG9490.1916745773666458No Hit
GACAGCAGTATCATCGCAGCAGGTGTGTAAGTCTTTTGGTTAGAAGACAA8920.1801619842055301No Hit
GACAGCAGTATCATCGAGTCATCATGGAGTCGCGAGCGCTATGAGCTATG8920.1801619842055301No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCCTATGGAGTCGTCAGCA8890.17955605824968188No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG8450.17066914423057503No Hit
GACAGCAGTATCATCGATGGATGGTCCATCGATGGTAAACTTATCTTTAT8140.16440790935347702No Hit
GACAGCAGTATCAAATAAACATAAATAAATTGTTTGTTGAATTTATTATT7910.1597624770253075No Hit
GACAGCAGTCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACAA7890.15935852638807538No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG7290.14724000727111147No Hit
GACAGCAGTATCATCGAAATCCAAGCATTTTCTAAATTAAATGTATTCTT7110.1436044515360223No Hit
GACAGCAGTATCATCGTCAGCAGCGGCAGCATCAGCTGTCAGCGAGAAAG7100.14340247621740623No Hit
GACAGCAGTATCATCCATCAGTCCAGTCTTTTGGTTAGAAGACAAATCCA6900.13936296984508492No Hit
GCCAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG6710.1355254387913797No Hit
GACAGCAGTATCATCGAGAGTCGAGAGCGCGCTCTATGGAGTCGTCAGCA6460.13047605582597807No Hit
GACAGCAGTATCATCGATACAGTATCACAGCAGTATTCCGACTATGGAGT6430.12987012987012986No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG6180.12482074690472825No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT5960.12037728989517482No Hit
GACAGCAGTATCATCGCAGCAGTATGGATCGACAAATGGCAGTATTCATC5880.1187614873462463No Hit
GACAGCAGTATCATCATCATCTATGGAGTCGAGAGCGCGCTATGGAGTCG5850.1181555613903981No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCTCCATACTATGGAGT5790.1169437094787017No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG5650.1141160550180768No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA5380.10866272141544303No Hit
GACAGCAGTATCATCAGTATGGAGTCGAGAGCGCGCTCCTATGGAGTCGT5240.10583506695481812No Hit
GACAGCAGCATCATCAGTATGTATATAAAAAATATATCCCGGTGTTTTAT5200.10502716568035386No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGCGCGCGCTCGTCGACTATGG5090.10280543717557714No Hit
GACAGCAGTATCATCGGGTCATATGGATCGACAAATGGCAGTATTCATCC5050.10199753590111288No Hit
GACGCAGTATCACGCGCGACCCGGGCTGCAGGAATTCGATAAAAGTTTTG5020.1013916099452647No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC5010.10118963462664862No Hit
GACAGCAGTATCATCGAAAGAAATGCCATCTAGTGATGATGAGGCTACTG4970.10038173335218435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC262700.0275.384289
AGTATCA288250.0273.29877
GTATCAT276700.0272.215588
GTATCCT10200.0250.696098
AGTATCC11050.0236.497747
TATCCTA9750.0234.887189
CAGCCTA9000.0224.83
ACAGCCT9000.0223.238892
GTATCAA7500.0222.926678
GCAGCTA7350.0214.095255
AGCAGCT7550.0210.284764
ACAGCTA8550.0187.333342
GTACTAT6650.0185.92488
GACAGCT9100.0182.186831
TATCACT4800.0178.552089
GTATCAC6800.0169.426478
CAGTCTA10700.0169.387856
CAGTATC503100.0163.846856
TATCATA10550.0162.473929
AGCAGTC13400.0155.179114
Produced by FastQC (version 0.11.5)