FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_WTA002-Lib2_1-C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_WTA002-Lib2_1-C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730630
Sequences flagged as poor quality0
Sequence length287
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG13156418.006925530022038No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG76161.042388076043962No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC66090.904561816514515No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG63970.8755457618767365No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC46740.6397218838536606No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG44270.6059154428370037No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA43680.597840220084037No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG42770.585385215498953No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA41920.5737514200073909No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT36910.5051804606983015No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG34290.4693209969478395No Hit
GACAGCAGTATCATCGAGCAGTATGGAGTCGACTACAGTCGCTACGACAT33490.4583715423675458No Hit
GACAGCAGTATCATCGACTCCATCCATACTCCACCTTGACTAGAGATCAT33250.45508670599345774No Hit
AACTCACGTAATGTCAAGAGAATTATAATATCCATACATAAATAATACAA32940.45084379234359384No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA30830.42196460588806917No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA27100.37091277390744976No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC26200.3585946375046193No Hit
GACAGCAGTATCATCGAGTCGCGATGCGCTATGGAGTCGCGAGCGCTATG24080.32957858286684094No Hit
GACAGCAGTATCATCATCATCTATGGAGTCGCGAGCGCTATGACAAATGG22390.30644786006597047No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG21510.2944034600276474No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT20920.2863282372746807No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT20720.28359087362960733No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT20410.2793479599797435No Hit
GACAGCAGTATCATCGGAGTCACTTCTGTGGTGTGACATAATTGGACAAA19860.27182020995579154No Hit
TAAAAACAAAGCTAGAGTTGTGGAATGCAAAGTTGGCGATTTCGTATTAT19570.2678510326704351No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC19010.26018641446422947No Hit
GACAGCAGTATCATCATCTAAATTAAATGTATTCTTATTATTATAGTTGT18620.2548485553563363No Hit
GACAGCAGTATCATCGATGGACCCTTCCATATCTCACCCTTGAGTGGCAC17530.2399299234906861No Hit
GACAGCAGTATCATCGAGTGAAATCCAAGCATTTTCTAAATTAAATGTAT17130.23445519620053928No Hit
GAGAAAGACGGCAAAATGGAATAAAAAATACCAGGCCGACATCGACATTT16650.22788552345236304No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC16570.22679057799433366No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG16480.22555876435405064No Hit
GACAGCAGTATCATCAGTGTATGCACGGATGCGTTTGAACTGACAGCCAC16450.22514815980728958No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG15280.20913458248361005No Hit
GACAGCAGTATCATCGAAAGATTCCAACCTATGGAACTGATGAATGGGAG14580.19955380972585304No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC14190.19421595061795985No Hit
GACAGCAGTATCATCGATACTGAATGGTGAATTATGAAGAGGGCGCGCGA14070.1925735324309158No Hit
GACAGCCTATGGAGTCCTATGGATCGACAAATGGCAGTATTCATCCACAG13830.18928869605682766No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA12840.1757387460137142No Hit
GACAGCAGTATCATCCAGTATAGTTCCAGTGAAATCCAAGCATTTTCTAA12430.17012715054131367No Hit
GACAGCAGTATCATCATACATTATACGAAGTTATGCTAGCCTAGTTCCAG12280.16807412780750858No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA12250.16766352326074757No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG12090.16547363234468884No Hit
TACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG12070.16519989598018148No Hit
GACAGCAGTATCATCATGTCACTTTCAGTTCAAATTGTAACGCTAATCAC12060.1650630277979278No Hit
GACATCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG12000.1642418187044058No Hit
GACAGCAGTATCATCATCATCACTATGGAGTCGAGAGCGCGCTCGTCGAT11950.16355747779313742No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA11730.16054637778355665No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC11450.15671406868045384No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG10590.1449434050066381No Hit
CAGCAACGACGGTTGGGCACATCCCGTCTCATCCCCACTCTCTCGAATTG10540.14425906409536976No Hit
GACAGCAGTATCATCATCATCAATTAGAATAAATATAGCTGTGAGGGAAA10490.1435747231841014No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG10280.1407004913567743No Hit
TTTTTAAGTGTATAATGTGTTAAACTACTGATTCTAATTGTTTGTGTATT10220.13987928226325227No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA10150.13892120498747657No Hit
GACAGCAGTATCATATATCTAGATATCAGAAGAAATGCCATCTAGTGATG9960.1363207095246568No Hit
GACAGCAGTATCACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTC9690.1326252686038077No Hit
GCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACT9470.1296141685942269No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA9450.12934043222971955No Hit
GACAGCAGTATCATCAGTACCACATTTGTAGAGGTTTTACTTGCTTTAAA9280.12701367313140713No Hit
GACAGCAGTATCATCCAAGCATTTTCTAAATTAAATGTATTCTTATTATT9120.12482378221534839No Hit
GACAGCAGTATCATCATCATCAGAGTGAGAGAGCATTAGTGCAGAGAGGG9010.123318232210558No Hit
GACAGCAGTATCATCGATGATGGAGTCGAGAGCGCGCTCGTCGACTATGG8930.12222328675252864No Hit
GACAGCAGTATCATCGATACTGGATACTGGATACGATACTCCATCGTATG8530.11674855946238179No Hit
GACAGCAGTATCATCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATC8400.11496927309308405No Hit
GCAGCAGCATCAGCAACAACAGCACCAGGGACACCAGCAGGCCACGGATC8240.11277938217702532No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACAGCAGTATCTGCTGTCATGG8200.11223190944801062No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC8130.11127383217223491No Hit
GACAGCAGTATCAGTATGGAGTCGACAGCAGTATCTGCTGTCATGGAGTC8110.11100009580772759No Hit
GACAGAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCGACAGAG8100.11086322762547392No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGCGCGCGCTCGTCGACTATGG8090.11072635944322023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8070.1104526230787129No Hit
GACAGCAGTATCATCGACTATGGAGTCCTATGGAGTCGTCAGCAGTACTA7880.10785212761589312No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGCGTATGGAGTCGACTAC7850.10744152306913211No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG7670.10497789578856602No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCATAGACATCGAGTATGG7550.10333547760152198No Hit
GACAGCAGTATCATCGACTAAGTATGGAGTCGACAGCAGTATCTGCTGTC7390.10114558668546322No Hit
GACAGCAGTCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGACA7320.10018750940968751No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC425500.0276.54239
AGTATCA470150.0271.885387
GTATCAT453550.0271.211038
GTATCCT16750.0247.447758
TCACGTA5450.0242.330284
CTCACGT5400.0241.972233
GATCGTG6350.0234.535423
TATCCTA16150.0232.281729
CGGATCG9100.0228.50551
CGTGCAA2800.0225.803576
CGTAATG5750.0224.87
ACAGCCT15600.0219.756422
CAGCCTA15450.0218.252443
AGTATCC18950.0217.237477
TATCACT9150.0210.366129
GTATCAC12450.0209.903638
GTAATGT6250.0209.0648
ACAGCTA15650.0205.587862
ACGTAAT6300.0205.174596
ACTCACG6400.0204.164052
Produced by FastQC (version 0.11.5)