FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_WTA002-Lib2_1-C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_WTA002-Lib2_1-C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917855
Sequences flagged as poor quality0
Sequence length287
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG16796518.299731439061727No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG114941.252267515021436No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC108981.187333511284462No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG72710.7921730556569393No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG60340.657402313001509No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA55790.6078302128331817No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA46890.5108650059105196No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG45650.49735524674376674No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA45260.4931062095864815No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA45170.492125662550185No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG44990.49016456847759177No Hit
GACAGCAGTATCATCGAAATATTTGATGTATAGTGCCTTGACTAGAGATC43830.4775264066764358No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC40960.44625785118564476No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT33990.37031993070800945No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA29860.32532371670906624No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT29800.32467001868486856No Hit
AACTCACGTAATGTCAAGAGAATTATAATATCCATACATAAATAATACAA29770.32434316967276966No Hit
CAGCAACGACGGTTGGGCACATCCCGTCTCATCCCCACTCTCTCGAATTG27040.2945999095717733No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC25380.27651426423563635No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC24650.26856093827456406No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA24360.2654013978242751No Hit
GACAGCAGTATCATCGACTACTGTCGACATAGCCATCGTAGCTATGGAGT24070.2622418573739861No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA23330.2541795817422142No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC22870.24916789689003166No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT21970.23936242652706582No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGATCGACAAATGGCAG21670.23609393640607723No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCTATGGAGTCGTCAGC20710.22563476801891363No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG20480.22312892559282238No Hit
GAGAAAGACGGCAAAATGGAATAAAAAATACCAGGCCGACATCGACATTT19130.20842072004837367No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG18680.20351798486689077No Hit
GACAGCAGTATCATCATGACCGTCAGTCGCGCGAGCGCGACCCGGGCTGC18640.20308218618409227No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG18600.20264638750129377No Hit
GACAGCAGTATCATCGAGCGCGCTCGTCGACTATGGAGTCGTCAGCATTG17050.18575918854285264No Hit
GACAGCAGTCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACAA16970.18488759117725567No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16630.18118330237346858No Hit
GACAGCAGTATCATCGCAGCAGGTGTGTAAGTCTTTTGGTTAGAAGACAA16390.1785685102766777No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCCTATGGAGTCGTCAGCA16290.1774790135696815No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG16140.17584476850918718No Hit
GACAGCAGTATCAAATAAACATAAATAAATTGTTTGTTGAATTTATTATT15850.17268522805889822No Hit
GACAGCAGTATCATCGATGGATGGTCCATCGATGGTAAACTTATCTTTAT15820.17235837904679932No Hit
GACAGCAGTATCATCGAGTCATCATGGAGTCGCGAGCGCTATGAGCTATG15220.1658213988048221No Hit
GACAGCAGTATCATCGTCAGCAGCGGCAGCATCAGCTGTCAGCGAGAAAG14320.1560159284418563No Hit
GACAGCAGTATCATCGATACAGTATCACAGCAGTATTCCGACTATGGAGT14160.15427273371066236No Hit
GACAGCAGTATCATCGAAATCCAAGCATTTTCTAAATTAAATGTATTCTT13320.14512096137189426No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG13170.14348671631139995No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG13160.14337776664070034No Hit
GACAGCAGTATCATCGAGAGTCGAGAGCGCGCTCTATGGAGTCGTCAGCA12980.14141667256810717No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCTCCATACTATGGAGT12970.14130772289740753No Hit
GACAGCAGTATCATCCATCAGTCCAGTCTTTTGGTTAGAAGACAAATCCA12810.13956452816621362No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT12560.1368407863987231No Hit
GACAGCAGTATCATCGCAGCAGTATGGATCGACAAATGGCAGTATTCATC12540.13662288705732387No Hit
GACAGCAGTATCATCAGTATGGAGTCGAGAGCGCGCTCCTATGGAGTCGT11620.1265995173529588No Hit
GACAGCAGTATCATCATCATCTATGGAGTCGAGAGCGCGCTATGGAGTCG11340.12354892657336944No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA11320.1233310272319702No Hit
GACAGCAGTATCATCGGGTCATATGGATCGACAAATGGCAGTATTCATCC10920.11897304040398539No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTATGGAGTCGTCAGCAGTACTACT10550.11494190258809943No Hit
GACAGCAGCATCATCAGTATGTATATAAAAAATATATCCCGGTGTTTTAT10180.11091076477221348No Hit
GCAGCAGCATCAGCAACAACAGCACCAGGGACACCAGCAGGCCACGGATC10170.11080181510151386No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACAGCAGTATCTGCTGTCATG10150.1105839157601146No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC10140.11047496608941498No Hit
GACGCAGTATCACGCGCGACCCGGGCTGCAGGAATTCGATAAAAGTTTTG9840.10720647596842638No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG9760.10633487860282943No Hit
GACAGCAGTATCATCGAAAGAAATGCCATCTAGTGATGATGAGGCTACTG9620.10480958321303474No Hit
GACAGCAGTACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGAC9450.10295743881114118No Hit
GACAGCAGTATCATCGATGGATTTTTTTAATAAATAAATAAACATAAATA9410.10252164012834271No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGAGCTCGTCGACTATGG9230.10056054605574954No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGCGCGCGCTCGTCGACTATGG9180.10001579770225145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC488700.0275.163829
AGTATCA539900.0273.713477
GTATCAT518200.0271.374888
GATCGTG5750.0237.017413
CGGATCG11100.0226.572071
GTATCAA16400.0225.314038
AGTATCC23600.0225.038137
GTATCCT22950.0222.840968
TATCCTA22300.0215.475349
CAGCCTA19400.0212.922673
TCACGTA5150.0212.796134
CGTGCAA3250.0211.830766
ACAGCCT19650.0210.928742
TATCACT9700.0205.68049
GGATCGT10600.0200.146222
ACTCACG5650.0193.96462
CGACGGT4100.0191.902447
ATCGTGC5800.0186.525864
GACAGCT21000.0184.657151
GTAATGT6050.0181.140498
Produced by FastQC (version 0.11.5)