FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_WTA002-Lib2-C9C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_WTA002-Lib2-C9C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253341
Sequences flagged as poor quality0
Sequence length287
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG4991519.702693207968704No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG26241.0357581283724309No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG25230.9958909138276079No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC25140.9923383897592573No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG17940.7081364642912122No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA16210.6398490571995847No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA15940.629191484994533No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG15930.6287967600980496No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC15750.6216917119613485No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT14870.5869559210708096No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA12720.5020900683268795No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG11660.4602492292996396No Hit
GACAGCAGTATCATCGACTCCATCCATACTCCACCTTGACTAGAGATCAT11450.4519600064734883No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA10460.4128822417216321No Hit
GACAGCAGTATCATCGAGCAGTATGGAGTCGACTACAGTCGCTACGACAT10320.4073560931708646No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC9260.3655152541436246No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT8460.3339372624249529No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT8410.33196363794253597No Hit
GACAGCAGTATCATCGAGTCGCGATGCGCTATGGAGTCGCGAGCGCTATG8330.32880583877066877No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG8190.3232796902199013No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC8030.3169640918761669No Hit
GACAGCAGTATCATCGGAGTCACTTCTGTGGTGTGACATAATTGGACAAA7960.3142010176007831No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT7610.30038564622386427No Hit
GACAGCAGTATCATCATCATCTATGGAGTCGCGAGCGCTATGACAAATGG7520.2968331221555137No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC6900.27236017857354317No Hit
TAAAAACAAAGCTAGAGTTGTGGAATGCAAAGTTGGCGATTTCGTATTAT6810.2688076545051926No Hit
GACAGCAGTATCATCATCTAAATTAAATGTATTCTTATTATTATAGTTGT6380.25183448395640656No Hit
GACAGCAGTATCATCGAGTGAAATCCAAGCATTTTCTAAATTAAATGTAT6380.25183448395640656No Hit
GACAGCAGTATCATCGATGGACCCTTCCATATCTCACCCTTGAGTGGCAC6350.2506503092669564No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG6240.24630833540563907No Hit
GACAGCCTATGGAGTCCTATGGATCGACAAATGGCAGTATTCATCCACAG6050.23880856237245449No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG5990.23644021299355414No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC5940.23446658851113714No Hit
GACAGCAGTATCATCAGTGTATGCACGGATGCGTTTGAACTGACAGCCAC5790.22854571506388624No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA5530.21828286775531794No Hit
GACAGCAGTATCATCGAAAGATTCCAACCTATGGAACTGATGAATGGGAG5450.21512506858345076No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA5040.19894134782763154No Hit
GACAGCAGTATCATATATCTAGATATCAGAAGAAATGCCATCTAGTGATG4900.19341519927686399No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG4830.19065212500148024No Hit
GACAGCAGTATCATCGATACTGAATGGTGAATTATGAAGAGGGCGCGCGA4680.18473125155422926No Hit
GACAGCAGTATCATCATCATCACTATGGAGTCGAGAGCGCGCTCGTCGAT4540.17920510300346174No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA4530.17881037810697833No Hit
GCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACT4310.17012643038434364No Hit
GACAGCAGTATCATCATACATTATACGAAGTTATGCTAGCCTAGTTCCAG4210.16617918141950966No Hit
GACAGCAGTATCATCCAGTATAGTTCCAGTGAAATCCAAGCATTTTCTAA4200.16578445652302626No Hit
GACAGCAGTATCACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTC4170.16460028183357608No Hit
GACCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTA4130.1630213822476425No Hit
GACAGCAGTATCATCATGTCACTTTCAGTTCAAATTGTAACGCTAATCAC3970.15670578390390816No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA3970.15670578390390816No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG3750.14802183618127346No Hit
TTTTTAAGTGTATAATGTGTTAAACTACTGATTCTAATTGTTTGTGTATT3730.14723238638830666No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC3610.1424956876305059No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3590.14170623783753913No Hit
GACAGCAGTATCATCATCATCAATTAGAATAAATATAGCTGTGAGGGAAA3530.13933788845863876No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG3490.13775898887270516No Hit
GACAGAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCGACAGAG3430.1353906394938048No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACAGCAGTATCTGCTGTCATGG3380.1334170150113878No Hit
GACAGCCGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG3360.13262756521842103No Hit
GACAGCAGTATCATCGATACTGGATACTGGATACGATACTCCATCGTATG3280.12946976604655386No Hit
GACAGCAGTATCATCATCATCAGAGTGAGAGAGCATTAGTGCAGAGAGGG3250.12828559135710366No Hit
GACAGCAGTCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGACA3220.12710141666765348No Hit
GACAGCAGTATCATCCAAGCATTTTCTAAATTAAATGTATTCTTATTATT3170.12512779218523648No Hit
GACCGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG3140.1239436174957863No Hit
GACAGCAGTATCAGTATGGAGTCGACAGCAGTATCTGCTGTCATGGAGTC3130.1235488925993029No Hit
GACAGCAGTATCATCGAGTCGCTCATAGATTTGAGCGAACCGAATCGCGC3110.12275944280633613No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC3060.12078581832391914No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGCGCGCGCTCGTCGACTATGG3010.11881219384150216No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCATAGACATCGAGTATGG2970.11723329425556857No Hit
GACAGCAGTATCATCGATGATGGAGTCGAGAGCGCGCTCGTCGACTATGG2950.1164438444626018No Hit
GACACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCAGCAGTACTACTGA2940.1160491195661184No Hit
GACAGCAGTATCATCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATC2930.115654394669635No Hit
GACAGCAGTATCATCAGTACCACATTTGTAGAGGTTTTACTTGCTTTAAA2870.11328604529073462No Hit
GACAGCAGTATCATCGACTATGGAGTCCTATGGAGTCGTCAGCAGTACTA2800.11052297101535086No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG2780.10973352122238406No Hit
GACAGCAGTATCATCGACTAAGTATGGAGTCGACAGCAGTATCTGCTGTC2770.10933879632590066No Hit
ACTATGGAGTCGAGAGCGCGCCTATGGAGTCGTCAGCAGTACTACTCTAT2760.10894407142941727No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGAGCTCGTCGACTATGG2740.10815462163645047No Hit
GACAGCAGTATCATCGAGTCATCATGGAGTCGCGAGCGCTATGAGCTATG2670.1053915473610667No Hit
GACAGCAGTATCATCGACTATGGAGCTATGGAGTCGTCAGCAGTACTACT2610.10302319798216632No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGCGTATGGAGTCGACTAC2550.10065484860326596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC153200.0277.790139
AGTATCA167300.0275.28937
GTATCAT162400.0272.608068
GTATCCT7150.0255.454538
TATCCTA6450.0252.682179
TATCACT3350.0247.447759
GTATCAC4300.0235.255818
AGTATCC8200.0227.884147
ACAGCCT5350.0225.850482
AAGCTAG1400.0220.78579
CAGCCTA5450.0219.128453
CAGCACT7100.0205.802833
ACAGCAC7750.0197.606432
GACACTA4650.0196.397841
GCAGCTA3750.0187.333345
GTATCTA4100.0185.048788
GTATCAA3200.0180.015628
TATCATA5100.0179.068629
AGCAGCT3950.0177.848114
ACAGCTA5650.0176.557512
Produced by FastQC (version 0.11.5)