FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n02_WTA002-Lib1-C9A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n02_WTA002-Lib1-C9A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109163
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG2379221.79493051675018No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA17051.5618845213121662No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG15541.423559264585986No Hit
GACAGCAGTATCATCGCGCGGAACTAACGACAGTCGCTCCAAGGTCGTCG12551.149656935042093No Hit
GACAGCAGTATCATCGTACGCGGGTGCTTACGACCGTCAGTCGCGCGAGC10730.982933777928419No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG7830.7172760001099274No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG7000.6412429119756694No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA6770.6201735020107546No Hit
GACAGCAGTATCATCGAAATGGGAGCAGTGGTGGAATGCCTTTAATGAGG6740.6174253181022874No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCTCTATGGAGTCGAGA6020.5514689042990757No Hit
GACAGCAGTATCATCGAGAGCCTTGACTAGAGATCATAATCAGCCATACC6000.549636781693431No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC5970.5468885977849638No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA5550.5084140230664236No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA5500.5038337165523117No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT5180.4745197548619954No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC5090.46627520313659393No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT5080.46535914183377153No Hit
GACAGCAGTATCATCCAAGCATTTTCTAAATTAAATGTATTCTTATTATT4990.45711459010837No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT4960.45436640619990293No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC4740.4342130575378104No Hit
GACAGCAGTATCATCTCCATCCATGGAGTCGCTATGGATCGACAAATGGC4720.4323809349321656No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG4680.4287166897208762No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGGTATGGA4340.397570605424915No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG4190.38382968588257926No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA3790.34718723376968386No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA3700.3389426820442824No Hit
GACAGCAGTATCATCGTACTGCATCGTACTGCAGTATCAGCAGAACTGAA3680.3371105594386376No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG3660.33527843683299285No Hit
GACAGCAGTATACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG3530.32336963989630185No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC3380.3096287203539661No Hit
GACAGCAGTATCATCGACTATGGCCATCTAGTGATGATGAGGCTACTGCT3340.30596447514267655No Hit
GACAGCAGTATCATCGAGCGCGACCCGGGCTGCAGGAATTCGATAAAAGT3210.29405567820598555No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA3000.2748183908467155No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC2880.26382565521284684No Hit
GACAGCAGTATCATGCTGCAATGTTGTTGTTGTTAACTTGTTTATTGCAG2820.25832928739591254No Hit
GACAGCAGTATCATCGACTACGATGGAGTCGACAGCAGTCTATGGAGTCG2810.25741322609309014No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG2710.2482526130648663No Hit
GACAGCAGCACTCCGACAGAGTGAGAGAGCATTAGTGCAGAGAGGGAGAG2620.2400080613394648No Hit
GACAGCAGTATCATCGTATGGAGTATGGAGTCGACAGAGATATCATGCAA2530.2317635096140634No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACAGCAGTATCTGCTGTCATG2440.2235189578886619No Hit
GACAGCAGTATCATCGATATGGAGTCGTCAGCAGTACTACTGACGATGGA2370.2171065287689052No Hit
GACAGCAGTATCATCGACCCCAAGGACTTTCCTTCAGAATTGCTAAGTTT2340.2143583448604381No Hit
GACAGCAGTATCATCACTATGGAGTCGAGAGCGCGCTCGTCGAAGTATGG2320.21252622225479328No Hit
GACAGCAGTATCATCGACATTACATTAGCTTTTTGAGGGGGCAATAAACA2230.2042816705293918No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT2230.2042816705293918No Hit
GACAGCAGTATCATCGAACCAACGAGAGCAGTATGCCGTTTACTGTGTGA2170.1987853027124575No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCATCTGCTGTCA2090.19145681228987843No Hit
GACAGCAGTATCATCTATGGAGTCCTATGGAGTCGTCAGCAGTACTACTG2070.18962468968423365No Hit
GACAGCAGTATCATCGAGTGAGAGGAGTCGAGAGCATAGACATCGAGTAT2060.18870862838141128No Hit
GACAGCAGTATCATCGACATGAATCGACATGACACAAGGGGTTGTGACCG2040.1868765057757665No Hit
GACAGCAGTATCATCGAGTCCATTTTTTGAGATGCATCTACACAAGGAAC2040.1868765057757665No Hit
GACAGCAGTATCATCGATCATGGAGTCGCGAGCGCTATGAGCGACTATGG2010.18412832186729935No Hit
GACAGCAGTATCATCCTATGGAGTCAGTATGGAGTCGACTACAGTCGCTA1990.18229619926165458No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG1950.17863195405036505No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTAATGCCTCACACTC1890.17313558623343075No Hit
GACAGCAGTATCATCAGCTTGTTCAGCTGCGCTTGTTTGTTTGCTTGGCT1890.17313558623343075No Hit
GACAGCAGTATCATCGATGTTTTTAAGCGCTCGATCGACTATGGAGTCGT1880.17221952493060835No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG1870.17130346362778595No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1840.1685552797193188No Hit
GACAGCTCTATGGAGTCGAGAGCATAGACATCGAGTATGGAGTCGACTAC1830.16763921841649643No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCTCCATACTATGGAGT1820.16672315711367405No Hit
GACAGCAGTATCATCGACGATGGAGTCGACAGCAGTGTGTGAGTCTATGG1750.16031072799391735No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCCTATGGAGTCGTCAGCAGTA1730.15847860538827258No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC1670.15298223757133828No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG1670.15298223757133828No Hit
GACAGCAGTATCATTCTATGGAGTCGAGAGCATAGACATCGAGTATGGAG1640.1502340536628711No Hit
GACAGCAGTATCACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTC1620.14840193105722635No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC1600.14656980845158157No Hit
GACAGCAGTATCATCGATGGTCGCAGTATCTATGGAGTCGCGAGCGCTAT1600.14656980845158157No Hit
GACAGCAGTATCATCATCGATGCCATGGATCGACAAATGGCAGTATTCAT1560.14290556324029205No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGGACTCCATCG1520.1392413180290025No Hit
GACAGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA1500.13740919542335775No Hit
GACAGCAGCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCGACAG1500.13740919542335775No Hit
GACAGCAGTATCATCGACCCATCGACTCCCATCGACCCGGGCTGCAGGAA1490.13649313412053535No Hit
TATGGAGTCGTCAGCAGTACCTATGGAACTGATGAATGGGAGCAGTGGTG1470.13466101151489057No Hit
GACAGCAGTAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCAGT1430.13099676630360105No Hit
GACAGCAACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG1420.13008070500077865No Hit
GACAGCAGTATCATCGACAGCAGTATCATCGGACAGTATGGAGTCGACTA1400.1282485823951339No Hit
GACACTATGGATCGACAAATGGCAGTATTCATCCACAGGATCTTTGTGAA1370.12550039848666675No Hit
GACAGCAGTATCATCGATACTATGGAGTCGAGAGCGCGCTATGGAGTCGT1360.12458433718384435No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTATGGAGTCGAGAGCATAGACAT1350.12366827588102196No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCATAGACATCGAGTATGG1350.12366827588102196No Hit
GACAGCAGTATCATCGTCGCGAGCGCTATGGATCGACAAATGGCAGTATT1340.12275221457819958No Hit
GACAGCCTATGGAGTCGAGAGCATAGACATCGAGTATGGAGTCGACTACA1340.12275221457819958No Hit
GACAGCAGTATCATCGACTATGGAGCTATGGAGTCGTCAGCAGTACTACT1320.1209200919725548No Hit
GACAGCAGTATCATCGACTACTATGGAGTCGTCAGCAGTACTACTGACGA1290.11817190806408764No Hit
GACAGCAGTATCATCGACTATGGAGAGTATGGAGTCGACTACAGTCGCTA1270.11633978545844288No Hit
GACAGCAGTATAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGTCG1260.1154237241556205No Hit
GACAGCAGTATCATCGACTATGGAGTCGACAGAGATATCATGCAGTATGG1250.1145076628527981No Hit
GACAGCCTATGGAGAGTATGGAGTCGACTACAGTCGCTACGACTATGGAG1250.1145076628527981No Hit
GACAGCAGTATCATCTGGATATCATCGTCAGCAGTACTACTGACGATGGA1210.11084341764150855No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGACTATGGAGTCGTCAGCAGT1210.11084341764150855No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGCTATGGAACAAA1200.1099273563386862No Hit
TATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTAC1190.10901129503586379No Hit
GACAGCAGTATCATCGACAAATGGCAGTATTCATCCACAGGATCTTTGTG1170.10717917243021904No Hit
GACAGCAGTATCATCAGCCATACCACATTTGTAGAGGTTTTACTTGCTTT1150.10534704982457425No Hit
GACAGCTATGGACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTCG1140.10443098852175188No Hit
GACAGCAGTATCATGAGAGCAGCAGTATCATCATCATGGAGTCGAGAGCG1140.10443098852175188No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCTATGGAGTCGTCAGCAG1130.10351492721892949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGTTA109.779755E-4281.05
TATCATC64100.0276.616249
AGTATCA70350.0274.20977
GTATCAT67350.0273.90728
GTATCAA2300.0244.347828
GCAGCTA1450.0242.241385
GTATACT700.0240.857138
GTATCAC900.0234.166668
TATCCTA3100.0226.61299
AGTATCC3450.0223.98557
GTATCCT3450.0223.98558
TATCAAC2400.0216.604169
GTACTAT2150.0215.651178
AGCAGCT1700.0214.882344
GACACTA1800.0210.751
AGCCTAA202.7637174E-5210.754
AGCAGTT202.7637174E-5210.754
GACAGCT2950.0209.559331
CAGCCTA2100.0207.404753
ACAGCCT2100.0207.404752
Produced by FastQC (version 0.11.5)