FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n01_WTA002-Lib4-C9G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n01_WTA002-Lib4-C9G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188591
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAAGGCACTATACATCAAATATT3320.17604233500007954No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTC250.002572443622.06
GAGTGAC250.002572443622.06
GGATGCG250.002572443622.09
CCCTAAC302.8603448E-422.01
TGGAAGT302.8603448E-422.02
GATCATG250.002572443622.01
TGAGTAT250.002572443622.06
GTAGGAC250.002572443622.08
CACTGGG302.8603448E-422.01
TGCGGAG302.8603448E-422.03
TGTGACT353.196834E-522.010
CCCGTAT302.8603448E-422.05
ACAGGAC302.8603448E-422.07
GCTTCAC302.8603448E-422.01
CCATCCA353.196834E-522.05
CTACGGG250.002572443622.01
ACTGTTG250.002572443622.05
GTCCGGC250.002572443622.07
CAGGACT250.002572443622.08
GAACGAG302.8603448E-422.03
Produced by FastQC (version 0.11.5)