FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n01_WTA002-Lib3-C9T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n01_WTA002-Lib3-C9T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495110
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTTCTTCCTCTTCTCGTCTTCTTCA5870.11855951202763021No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGGA403.6084075E-622.00000210
ATCAGGA403.6084075E-622.0000027
TAAGGAG403.6084075E-622.0000029
CGGGTAG504.6100467E-822.03
TGAAGGG250.002578306722.05
ATTGTGT605.966285E-1022.03
GTGGCGG302.869461E-422.02
GAGACGA605.966285E-1022.07
GTATGCG250.002578306722.08
GGACGAA302.869461E-422.02
CGTACGA454.0717532E-722.07
CTGGGCG302.869461E-422.09
ACGAGGG454.0717532E-722.05
AAAGATT250.002578306722.06
CTAGCCG250.002578306722.06
GAGGCGC250.002578306722.07
AAGGCAG1400.021.2142877
CAATCGG1000.020.99
ATGGTGG1950.020.8717965
CGCGTGT950.020.84210610
Produced by FastQC (version 0.11.5)