FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n01_WTA002-Lib2_1-C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n01_WTA002-Lib2_1-C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730630
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAC504.6176865E-822.010
ACGGGAG302.8712724E-422.03
TGGGACC504.6176865E-822.011
GCTCCCG353.2131076E-522.06
CGACACG2150.021.488375
CGTAGCT3800.021.4210537
TCGGGGT1600.021.3125023
ATCGGGG1600.021.3125022
CCGAGGA900.020.7777795
GCCTCGT3350.020.6865673
CTGCGGC752.1827873E-1120.5333339
TAGGGCA2200.020.57
CGGGTGT1400.020.4285721
TTAGCGA701.8371793E-1020.4285726
CGGGGTC701.8371793E-1020.4285724
GGAACTA2050.020.3902442
GCCCGAA2700.020.370371
GCCGGAT1300.020.3076939
GACAGCC1850.020.2162171
CCAGCCG1850.020.2162176
Produced by FastQC (version 0.11.5)