FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n01_WTA002-Lib2_1-C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n01_WTA002-Lib2_1-C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917855
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAG403.6139736E-622.0000023
CGGGAGT250.002579969422.03
GAGGCGC605.984475E-1022.07
CGAGGCG656.91216E-1121.9999986
GGGATAG1200.021.0833323
CCGGGTA752.1827873E-1120.5333351
TGACTAG3600.020.4722233
GCGGTGT651.6225385E-920.3076925
AGATGCG5250.020.1142869
TGCGGAG1150.020.0869583
GATGGGT1650.020.03
GGGATCG3300.020.04
CGGGAAG2150.019.9534873
GGATCGC3200.019.9375025
CGCTGAG1600.019.9375023
GATGCGA5300.019.92452810
AGCGGAA2550.019.84313610
TAGCGTG501.122833E-619.87
GTATGCG950.019.684218
GCGGAAT950.019.6842111
Produced by FastQC (version 0.11.5)