FastQCFastQC Report
Wed 12 Aug 2020
000000000-G59KY_l01_n01_WTA002-Lib2-C9C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59KY_l01_n01_WTA002-Lib2-C9C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253341
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCCGC555.2041287E-922.00000210
TAGAGTG302.8641062E-422.04
AGCGTGG250.002574862422.02
GATGTGT302.8641062E-422.03
GCGACAG302.8641062E-422.09
GGTCGCT250.002574862422.06
CCGATTC250.002574862422.03
AGGAGAG403.596897E-622.03
ACTTGGT454.0555278E-722.03
GCTGGGT250.002574862422.01
TGGCGTG403.596897E-622.01
GGACTGA250.002574862422.010
GGACTCT250.002574862422.02
GCACGTG750.022.01
TCGGGGT605.9117156E-1022.03
GCCGGAT454.0555278E-722.09
TTGTACT403.596897E-622.06
CTAAGCG403.596897E-622.05
AGTATAC504.587673E-822.010
ACTAGGA504.587673E-822.05
Produced by FastQC (version 0.11.5)