FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212282
Sequences flagged as poor quality0
Sequence length287
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG26461.246455186968278No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13050.6147483064979603No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC3160.1488585937573605No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCTAT158.781108E-6281.05
AGTTTGC109.786255E-4280.999976
GTAGTTT109.786255E-4280.999974
TATCATC8350.0274.269479
AGTATCA9700.0263.618567
GACAGCC252.393881E-7224.799991
CAGCCTA252.393881E-7224.799993
AGCCTAT252.393881E-7224.799994
GTATCAC202.7673848E-5210.749988
GCAGTCT355.304173E-9200.71435
GTTTGCC150.0032940672187.333337
ACAGCCT305.939128E-7187.333332
GTATCAA305.939128E-7187.333338
TTGCCCT150.0032940672187.333339
TATAATC150.0032940672187.333339
GGTAGTT150.0032940672187.333333
CAGTCTA401.1783413E-8175.624986
CAGCTCT256.7371315E-5168.599993
CTCTATG256.7371315E-5168.599996
CAGTATC16100.0164.93486
Produced by FastQC (version 0.11.5)