FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n02_WTA002-Lib4-C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n02_WTA002-Lib4-C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320832
Sequences flagged as poor quality0
Sequence length287
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG3262310.168250049870336No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA27890.8693023139836424No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC19820.6177688011170955No Hit
GACAGCAGTATCATCGATGATGGATGATGGACTCCATCCATCCATCAGCC18570.5788076002393776No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG16610.5177164372631159No Hit
GACAGCAGTATCATCGAGGTTTTTTGTAGAGGTTTTACTTGCTTTAAAAA13720.42763814083383206No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG11950.3724690803909834No Hit
GACAGCAGTATCATACTTGTCGAGATACTTGTCGTGGATACTTGACGTAT11290.3518975663275484No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT11160.3478456014362657No Hit
GACAGCAGTATCATCGAAATATTTGATGTATAGTGCCTTGACTAGAGATC10730.33444294833433075No Hit
GACAGCAGTATCATCGTCGTTTGTTGAATTTATTATTAGTATGTAAGTGT10580.3297676042290046No Hit
GACAGCAGTATCATCAGCAGTATGGAGTCGACAGCAGTATCTGCTGTCAT10340.32228705366048277No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA10050.31324805505685216No Hit
GACAGCAGTACCAGTGAAATCCAAGCATTTTCTAAATTAAATGTATTCTT9390.2926765409934171No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA9250.2883128864951127No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC8640.26929982046678635No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC7860.24498803111909034No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT7620.23750748055056853No Hit
GACAGCAGTATCATCGAAACTTTTTTGTATCATTTTGAAGCATCATTTTT7600.23688410133652504No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG7560.23563734290843805No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG7530.23470227408737285No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7400.23065030919609017No Hit
GACAGCAGTATCATCGACCTACCACAATAACCAGTTTGTTTTGGGATTCT6890.21475413923798126No Hit
GACAGCAGTATCATCGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAG6880.21444244963095951No Hit
GACAGCAGTATCATCGACTACTGTCGACATAGCCATCGTAGCTATGGAGT6670.2078969678835029No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA6660.20758527827648113No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGACGACTATGG6610.20602683024137244No Hit
AACTCACGTAATGTCAAGAGAATTATAATATCCATACATAAATAATACAA6360.19823459006582883No Hit
GACAGCAGTATCATCGAGAACTCTTGCTTGCTTTGCTATTTACACCACAA6240.19449431478156792No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA6220.19387093556752444No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT6170.19231248753241573No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA6140.19137741871135047No Hit
GACAGCAGTATCATCGATGATACCATCGATATGGAGTCGAGAGCGCGCTA6140.19137741871135047No Hit
GACAGCAGTATCATCGGGCTGCAGGAATTCGATAAAAGTTTTGTTACTTT5880.18327348892878517No Hit
GACAGCAGTATCATCCATCACAAATAAAGCATTTTTTTCACTGCATTCTA5750.1792215240375025No Hit
GACAGCAGTATCATCGATCATGGATCGACAAATGGCAGTATTCATCCACA5600.17454617993217633No Hit
GACAGCAGTATCATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACA5570.1736111111111111No Hit
GACAGCAGTATCATCATCATTCACCTAGTTCCAGTGAAATCCAAGCATTT5450.1698708358268502No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC5440.16955914621982843No Hit
GACAGCAGTATCATCTATGATGATAAATAAATAAATTTAATAAATTAAAT5150.1605201476161979No Hit
GACAGCAGTATCATCGAAGTAGATCTTTAGATACACCCTAATACTAAATA5140.16020845800917616No Hit
GACAGCAGTATCATCTTATTATTATAGTTGTTATTTTTGATATATATAAA5140.16020845800917616No Hit
GACAGCAGTATCATCGATGCAGTTGACGATCATCATCATCATCATCATCA5030.15677987233193696No Hit
GCAGCAGCATCAGCAACAACAGCACCAGGGACACCAGCAGGCCACGGATC4890.15241621783363257No Hit
GACAGCAGTAAGAGAAAGGTAGAAGACCCCAAGGACTTTCCTTCAGAATT4850.15116945940554558No Hit
GACAGCAGTATCATCAAACACTGGATGTCACTTTCAGTTCAAATTGTAAC4850.15116945940554558No Hit
GACAGCAGTATCATCGAATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG4810.1499227009774586No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG4750.14805256333532815No Hit
GACAGCAGTATCATCGATACTTCATCGATGTCTATGCTCTCGACTCCATA4690.14618242569319767No Hit
GACAGCAGTATCATCGATGATATCGCGCAGACCGCGCTAAATGTCGATGT4680.14587073608617593No Hit
GACAGCAGTATCATCGACTACAGAGATTTAAAGCTCTAAGATTCCAACCT4630.14431228805106722No Hit
GAAAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG4410.13745511669658886No Hit
GAGAAAGACGGCAAAATGGAATAAAAAATACCAGGCCGACATCGACATTT4390.13683173748254537No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG4270.13309146219828447No Hit
GACAGCAGTATCATCGGAGTGACTGGATGGATACTGGATATAAATATCCA4240.13215639337721924No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC4180.13028625573508879No Hit
GACAGCAGTATCATCGATCGTAGCGACTGTAGTCGACTCCATACTCGATG4140.12903949730700182No Hit
GACAGCAGTATCATAGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG4080.12716935966487133No Hit
GACAGCAGTATCATCGATACTGGATACTGGATACGATACTCCATCGTATG4060.12654598045082785No Hit
GACAGCAGTATCAAGTATGGAGTCGACTACAGTCGCTACGACTATGGAGT3930.12249401555954517No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG3840.11968880909634949No Hit
GACAGCAGTATCATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA3780.11781867145421902No Hit
GACAGCAGTATCATCTATGGAGTCGTCAGCAGTACTACTGACGATGGAGT3770.11750698184719728No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGGCATAGACAT3700.11532515459804508No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA3690.11501346499102334No Hit
GACAGCAGTATAATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG3650.11376670656293637No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG3620.11283163774187113No Hit
GACAGCAGTATCATCGATCACCTCCCCCTGAACCTGAAACATAAAATGAA3560.11096150009974068No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGAGCTCGTCGACTATGG3470.108156293636545No Hit
CTATGGAGTCGAGAGCGCACTATGGAGTCGTCAGCAGTACTACTGACGAT3360.10472770795930579No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGGAGTCGACAGAGATATCAT3330.10379263913824056No Hit
GACAGCAGTATCATCGATGCATCTACACAAGGAACAAACACTGGATGTCA3330.10379263913824056No Hit
GACAGCAGTATCATCGCTATGGAGTCGCGAGCGCTATGAGCGACTATGGA3260.10161081188908837No Hit
GACAGCAGTATCATACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG3230.10067574306802313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC155450.0277.29439
AGTATCA179550.0272.157627
GTATCAT171900.0270.78338
GATCGTG1000.0238.853
TATCCTA6250.0233.791999
GTACCAG1750.0224.88
ACTCACG1250.0224.82
CAGCCTA4400.0220.329563
TATAATC4150.0220.060239
TCACGTA1300.0216.153844
ACAGCCT4550.0213.065932
CGGATCG1650.0212.878781
GTATCCT7050.0211.248218
GACACTA4500.0206.066671
AGTATCC7600.0205.203957
CGTGCAA550.0204.363656
AGTACCA2600.0199.942297
GTATCAC3450.0199.550728
GTATCAA4850.0196.989698
TATCACT2150.0189.511639
Produced by FastQC (version 0.11.5)