FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n02_WTA002-Lib3-C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n02_WTA002-Lib3-C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185218
Sequences flagged as poor quality0
Sequence length287
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG2668314.406267209450485No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG20001.079808657905819No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC17630.9518513319439795No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG10940.590655335874483No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG8190.44218164541243293No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA7430.4011489164120118No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA7430.4011489164120118No Hit
GACAGCAGTATCATCGAAATATTTGATGTATAGTGCCTTGACTAGAGATC7350.3968296817803885No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG7270.3925104471487652No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA7190.38819121251714195No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA7140.3854916908723774No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG5960.3217829800559341No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC5920.31962336274012243No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT5560.3001868068978177No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5400.29154833763457116No Hit
AACTCACGTAATGTCAAGAGAATTATAATATCCATACATAAATAATACAA4730.25537474759472617No Hit
GACAGCAGTATCATCGACTACTGTCGACATAGCCATCGTAGCTATGGAGT4180.22568000950231618No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGACGACTATGG4140.22352039218650455No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA4140.22352039218650455No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC4110.22190067919964584No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC3990.2154218272522109No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCTATGGAGTCGTCAGC3560.1922059411072358No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA3530.19058622812037707No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC3520.19004632379142414No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGATCGACAAATGGCAG3440.18572708915980088No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG3390.18302756751503632No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA3350.1808679501992247No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT3270.17654871556760143No Hit
GACAGCAGTATCATCATGACCGTCAGTCGCGCGAGCGCGACCCGGGCTGC3260.1760088112386485No Hit
CAGCAACGACGGTTGGGCACATCCCGTCTCATCCCCACTCTCTCGAATTG3200.17276938526493105No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT3140.1695299592912136No Hit
GAGAAAGACGGCAAAATGGAATAAAAAATACCAGGCCGACATCGACATTT3130.1689900549622607No Hit
GACAGAAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG3080.16629053331749616No Hit
GACAGCAGTATCAAATAAACATAAATAAATTGTTTGTTGAATTTATTATT3020.16305110734377867No Hit
GACAGCAGTATCATCGCAGCAGGTGTGTAAGTCTTTTGGTTAGAAGACAA2910.15711215972529666No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG2900.15657225539634376No Hit
GACAGCAGTCTATGGAGTCGCGAGCGCTATGAGCGACTATGGATCGACAA2800.15117321210681467No Hit
GACAGCAGTATCATCGATGGATGGTCCATCGATGGTAAACTTATCTTTAT2790.15063330777786177No Hit
GACAGCAGTATCATCGAGCGCGCTCGTCGACTATGGAGTCGTCAGCATTG2640.14253474284356812No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG2560.13821550821194487No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCCTATGGAGTCGTCAGCA2540.137135699554039No Hit
GACAGCAGTATAATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG2540.137135699554039No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCTCCATACTATGGAGT2540.137135699554039No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG2500.13497608223822738No Hit
GACAGCAGTATCATCGATACAGTATCACAGCAGTATTCCGACTATGGAGT2480.13389627358032155No Hit
GACAGCAGTATCATAGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG2370.12795732596183956No Hit
GACAGCAGTATCATCGAGTCATCATGGAGTCGCGAGCGCTATGAGCTATG2360.12741742163288666No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG2190.11823904804068718No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGAGCTCGTCGACTATGG2170.11715923938278136No Hit
GACAGCAGTATCATCGAAATCCAAGCATTTTCTAAATTAAATGTATTCTT2110.11391981340906392No Hit
GACAGCAGTATCATCGCAGCAGTATGGATCGACAAATGGCAGTATTCATC2080.1123001004222052No Hit
GACAGCAGTATCATCGACTATGGAGACGAGAGCGCGCTCGTCGACTATGG2050.11068038743534646No Hit
GACAGCAGTATCATCGTCAGCAGCGGCAGCATCAGCTGTCAGCGAGAAAG2010.10852077011953483No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT1930.10420153548791153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGC207.8647645E-8281.08
TATCATC89650.0275.984959
AGTATCA100450.0274.42617
GTATCAT96600.0271.40068
ACTCACG500.0252.900012
GATCGTG700.0240.857153
GTATCAA2750.0240.127278
CGGATCG1750.0232.828581
ATCACGC252.3930625E-7224.89
CGTGCAA404.7293724E-11210.756
TATCACT1250.0202.329
AGTATCC4100.0195.329277
TCACGTA650.0194.538454
ACAGCCT3150.0191.793662
GTATCCT3900.0190.935888
TATAATC2800.0190.678579
GTACTAT2700.0187.333348
TTAGGCA150.0032937299187.333337
ATTAGGC150.0032937299187.333336
CGTGCTA150.0032937299187.333336
Produced by FastQC (version 0.11.5)