FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n02_WTA002-Lib2-C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n02_WTA002-Lib2-C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122620
Sequences flagged as poor quality0
Sequence length287
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG1855515.132115478714727No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG10330.8424400587179905No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC9290.7576251834937204No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG7600.6198010112542814No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG5560.45343337139129014No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC5500.44854020551296686No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA5490.44772467786657966No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA5180.42244332082857605No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG5010.40857935083999347No Hit
GACAGCAGTATCATCGAGCAGTATGGAGTCGACTACAGTCGCTACGACAT4710.38411352144837707No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT4620.3767737726308922No Hit
AACTCACGTAATGTCAAGAGAATTATAATATCCATACATAAATAATACAA4570.3726961343989561No Hit
GACAGCAGTATCATCGACTCCATCCATACTCCACCTTGACTAGAGATCAT4380.35720110911759906No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG3770.3074539226879791No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA3610.29440548034578373No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA3570.2911433697602349No Hit
GACAGCAGTATCATCGAGTCGCGATGCGCTATGGAGTCGCGAGCGCTATG3520.2870657315282988No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC3510.28625020388191164No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG2780.22671668569564507No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT2720.22182351981732182No Hit
GACAGCAGTATCATCATCATCTATGGAGTCGCGAGCGCTATGACAAATGG2700.22019246452454738No Hit
GACAGCAGTATCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTC2680.21856140923177297No Hit
GACAGCAGTATCATCATCTAAATTAAATGTATTCTTATTATTATAGTTGT2660.21693035393899854No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC2600.21203718806067526No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT2590.21122166041428803No Hit
GACAGCAGTATCATCGAGTGAAATCCAAGCATTTTCTAAATTAAATGTAT2530.20632849453596477No Hit
GACAGCAGTATCATCGATGGACCCTTCCATATCTCACCCTTGAGTGGCAC2500.20388191159680316No Hit
GACAGCAGTATCATCGGAGTCACTTCTGTGGTGTGACATAATTGGACAAA2460.2006198010112543No Hit
GACAGCAGTATCATCGAAAGATTCCAACCTATGGAACTGATGAATGGGAG2450.19980427336486706No Hit
TAAAAACAAAGCTAGAGTTGTGGAATGCAAAGTTGGCGATTTCGTATTAT2380.19409557984015657No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA2180.17778502691241233No Hit
GACAGCAGTATCATCAGTGTATGCACGGATGCGTTTGAACTGACAGCCAC2100.17126080574131464No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG2050.16718316750937856No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG2050.16718316750937856No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT2040.16636763986299136No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGACGACTATGG2010.16392105692382972No Hit
GACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTAC1980.16147447398466808No Hit
GACAGCAGTATCATCATCATCACTATGGAGTCGAGAGCGCGCTCGTCGAT1940.15821236339911923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.15250366987440872No Hit
GAGAAAGACGGCAAAATGGAATAAAAAATACCAGGCCGACATCGACATTT1860.15168814222802152No Hit
GACAGCCTATGGAGTCCTATGGATCGACAAATGGCAGTATTCATCCACAG1780.14516392105692383No Hit
GACAGCAGTATCATCGAGTATGGAGTCGACTACAGTCGCTACGACTATGG1690.1378241722394389No Hit
GACAGCAGTATCATCATCATCAATTAGAATAAATATAGCTGTGAGGGAAA1640.13374653400750286No Hit
GACAGCAGTATCATCGATACTGAATGGTGAATTATGAAGAGGGCGCGCGA1580.12885336812917958No Hit
GACAGCAGTATCATCAGTACCACATTTGTAGAGGTTTTACTTGCTTTAAA1550.12640678519001794No Hit
GACAGCAGTATCATCATGTCACTTTCAGTTCAAATTGTAACGCTAATCAC1500.12232914695808188No Hit
GACAGCAGTATCATCCAGTATAGTTCCAGTGAAATCCAAGCATTTTCTAA1490.12151361931169466No Hit
GACAGCAGTATCACTATGGAGTCGTCAGCAGTACTACTGACGATGGAGTC1480.12069809166530746No Hit
GACAGCAGTATCATCGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGA1460.11906703637253302No Hit
GACAGCAGTATCATCATACATTATACGAAGTTATGCTAGCCTAGTTCCAG1430.1166204534333714No Hit
GACAGCAGTATCATATATCTAGATATCAGAAGAAATGCCATCTAGTGATG1410.11498939814059697No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA1410.11498939814059697No Hit
GACAGCAGTATCTGCTGTCATGGAGTCGACTGCACGACAGTCGACTATGG1380.11254281520143533No Hit
GACAGCAGTATCATCGACTATGGAGTCGTCAGCAGTACTACTGACGATGG1380.11254281520143533No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC1350.11009623226227369No Hit
GACAGCAGTATCATAGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG1350.11009623226227369No Hit
GACAGCAGTATCATCCAAGCATTTTCTAAATTAAATGTATTCTTATTATT1330.10846517696949927No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGAGCTCGTCGACTATGG1320.10764964932311205No Hit
GACAGCAGTATCATCGACTATGGAGACGAGAGCGCGCTCGTCGACTATGG1270.103572011091176No Hit
GACAGCAGTATCATCATCATCAGAGTGAGAGAGCATTAGTGCAGAGAGGG1240.10112542815201436No Hit
TTTTTAAGTGTATAATGTGTTAAACTACTGATTCTAATTGTTTGTGTATT1240.10112542815201436No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCATAGACATCGAGTATGG1230.10030990050562714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAA207.8540324E-8280.999976
TATCATC59750.0276.061959
AGTATCA67400.0271.410957
GTATCAT65050.0267.392768
CGGATCG750.0262.266661
ACAGCCT2050.0260.439032
CAGCCTA2100.0254.23813
TATCACT1550.0253.806469
TCACGTA500.0252.900014
CGTAATG450.0249.777777
GTATCAC1800.0234.166668
TCGTACA302.1045707E-9234.166665
GACACTA1950.0216.153841
GTATCTA1800.0210.758
ACTCACG600.0210.752
TATCCTA2650.0206.773579
AGTATCC3200.0206.359367
GTATCAA1650.0204.363638
GACAGCC3150.0196.253981
GTATCCT3250.0194.538478
Produced by FastQC (version 0.11.5)