FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n02_WTA002-Lib1-C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n02_WTA002-Lib1-C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348407
Sequences flagged as poor quality0
Sequence length287
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGACTATGG4293812.324092225471933No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90732.6041382635825343No Hit
GACAGCAGTATCAACAACCTGTTTTGCTCAGAAGAAATGCCATCTAGTGA26370.7568734267681189No Hit
GACAGCAGTATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG24080.6911457002873077No Hit
GACAGCAGTATCATCGTACGCGGGTGCTTACGACCGTCAGTCGCGCGAGC17970.515776089458593No Hit
GACAGCAGTATCATCGCGCGGAACTAACGACAGTCGCTCCAAGGTCGTCG13390.3843206364969705No Hit
GACAGCAGTATCATCGAAATGGGAGCAGTGGTGGAATGCCTTTAATGAGG12470.3579147376487843No Hit
GACAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAG11980.34385072630572866No Hit
GACAGCAGCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAG10550.30280677483517837No Hit
GACAGCAGTATCATCGAGTCGAGAGCGCGCTCGTCTCTATGGAGTCGAGA10410.29878848588001966No Hit
GACAGCACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGC10170.2918999905283189No Hit
GACAGCAGTATCATCGAGAGCCTTGACTAGAGATCATAATCAGCCATACC10150.29132594924901045No Hit
GACAGCAGTATCATCGATATGGAGTCGAGAGCGCGCTCGTCGACTATGGA9900.28415043325765554No Hit
GACAGCAGTATCATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT9380.2692253599956373No Hit
GACAGCAGTCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCA8920.2560224105715442No Hit
GACAGCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCA8700.24970795649915187No Hit
GACAGCAGTATCATCGATGATGGAGTCCGACTCCGACATCATATGGAGTC8200.23535692451644197No Hit
GACAGCAGTATCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGT7980.22904247044404966No Hit
GACAGCAGTATCATCTCCATCCATGGAGTCGCTATGGATCGACAAATGGC7880.22617226404750765No Hit
GACAGCAGTACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC7720.22157993381304047No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGTCGGTATGGA7630.21899674805615274No Hit
GACAGCAGTATCATCTATTTATACTCCGGCGCTCTTTTCGCGAACATTCG7390.212108252704452No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTATGGAGTCGTCA6840.19632211752347112No Hit
GACAGCAGTATCATCCAAGCATTTTCTAAATTAAATGTATTCTTATTATT6740.19345191112692914No Hit
GACAGCAGTATACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCG6720.19287786984762073No Hit
GACAGCAGTATCATCGACTATGGAGTCGCGAGCGCTATGAGCGACTATGG6430.184554271297649No Hit
GACAGCAGTATCATCGTACTGCATCGTACTGCAGTATCAGCAGAACTGAA6420.18426725065799482No Hit
GACAGCAGTATCATCCTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAG6410.1839802300183406No Hit
GACACTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGT6100.17508259018906047No Hit
CTATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTCAGCAGTACTA5890.16905515675632235No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCGACGACTATGG5700.16360176460289258No Hit
GACAGCAGTATCATCGACTATGGCCATCTAGTGATGATGAGGCTACTGCT5640.16187964076496741No Hit
GACAGCAGTATCATCGAGCGCGACCCGGGCTGCAGGAATTCGATAAAAGT5570.15987049628738803No Hit
GACAGCAGTATCATCGATCTATGGATCGACAAATGGCAGTATTCATCCAC5560.1595834756477338No Hit
GACAGCAGTATCATCGATATGGAGTCGTCAGCAGTACTACTGACGATGGA5080.14580648494433235No Hit
GACAGCAGTATCATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGTCGTC4940.14178819598917358No Hit
GACAGCAGTATCATCGACTCCATCTATGGATCGACAAATGGCAGTATTCA4830.1386309689529774No Hit
GACAGCAGTATCATCGAGTGAGAGGAGTCGAGAGCATAGACATCGAGTAT4690.13461267999781865No Hit
GACAGCAGTATCATCGTATGGAGTATGGAGTCGACAGAGATATCATGCAA4690.13461267999781865No Hit
GACAGCAGTATCATCGACTACGATGGAGTCGACAGCAGTCTATGGAGTCG4400.1262890814478469No Hit
GACAGCAGTATCATGCTGCAATGTTGTTGTTGTTAACTTGTTTATTGCAG4350.12485397824957593No Hit
GACAGCAGTATCATCACTATGGAGTCGAGAGCGCGCTCGTCGAAGTATGG4180.11997462737545457No Hit
GACAGCAGTATCATCGAACTATGGAGTCGAGAGCGCGCTCGTCGACTATG4150.11911356545649197No Hit
GACAGCAGTATCATCGACTATGGAGTCGAGAGCGCGCTCATCTGCTGTCA3930.11279911138409963No Hit
GACAGCAGTATCATCGAGTCCATTTTTTGAGATGCATCTACACAAGGAAC3910.11222507010479124No Hit
GACAGCAGTATCATCGACCCCAAGGACTTTCCTTCAGAATTGCTAAGTTT3760.10791976050997826No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACAGCAGTATCTGCTGTCATG3740.10734571923066989No Hit
GACAGCAGTATCATCGATGGAGTCGAGAGCGCGCTCGTCGACTATGGAGT3720.1067716779513615No Hit
GACAGCAGCACTCCGACAGAGTGAGAGAGCATTAGTGCAGAGAGGGAGAG3720.1067716779513615No Hit
GACAGCAGTATCATCGAAGTATGGAGTCGACTACAGTCGCTACGACTATG3700.10619763667205309No Hit
GACAGCAGTATCATCTATGGAGTCCTATGGAGTCGTCAGCAGTACTACTG3680.1056235953927447No Hit
GACAGCAGTATCATCGAACCAACGAGAGCAGTATGCCGTTTACTGTGTGA3650.1047625334737821No Hit
GACAGCAGTATCATCGATCATGGAGTCGCGAGCGCTATGAGCGACTATGG3580.10275338899620272No Hit
GACAGCAGTATCATCGAGAGCGCGCTCGTCCTATGGAGTCGTCAGCAGTA3530.10131828579793171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC122700.0275.389169
GTATCAT132150.0269.730228
AGTATCA141400.0268.082737
GCAGCTA3800.0225.539475
GTATCAA6750.0220.637048
TATCCTA5750.0210.139149
AGTATCC6400.0208.554677
CAGCCTA3000.0206.066653
ACAGCCT3100.0199.419342
GTATCAC2500.0196.78
GTATCCT6550.0193.053448
TATCACT2300.0189.369559
AGCAGCT4700.0182.351074
GACAGCT5900.0171.457631
GTATCTA4500.0168.68
GTACTAT3300.0166.045448
CAGTATC246750.0162.393526
TATCATA6250.0155.1129
GTATACT2300.0152.717398
AGCCTAT4000.0151.03754
Produced by FastQC (version 0.11.5)