FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n01_WTA002-Lib4-C8G.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n01_WTA002-Lib4-C8G.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences320832
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTCAAGGCACTATACATCAAATATT4630.14431228805106722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGC302.8664118E-422.0000026
TATAGCG302.8664118E-422.0000025
ATCGCAC302.8664118E-422.0000024
AGTCGTG302.8664118E-422.0000024
TTGGGCG302.8664118E-422.0000021
GAAATGA250.00257634622.01
TCCGCAC353.20587E-522.08
CACGGGT403.6018519E-622.01
ACACCGT250.00257634622.07
CTGAGAT250.00257634622.09
ATAGCGA250.00257634622.06
AGACGCA250.00257634622.08
TGCGACG353.20587E-522.01
GATGAGC250.00257634622.04
CGTCGGT250.00257634622.010
CTCTGCG250.00257634622.07
AAGGGAA250.00257634622.02
TGGGATG250.00257634622.010
CGATAGA250.00257634622.04
GTAACGG403.6018519E-622.010
Produced by FastQC (version 0.11.5)