FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n01_WTA002-Lib3-C8C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n01_WTA002-Lib3-C8C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185218
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAAAG302.8600782E-422.03
CGGACAC250.002572271722.01
CATGGTG555.182301E-922.04
GGTACAT403.5882476E-622.09
GCCCACA454.0433406E-722.010
CAAAAGT250.002572271722.04
TGGACCA250.002572271722.09
GACGTAC250.002572271722.05
GATCCGC302.8600782E-422.06
GGATCGC555.182301E-922.05
CATTCGT250.002572271722.03
ACTTACC250.002572271722.02
CGTTCGG353.1964384E-522.07
TCTAGAC250.002572271722.010
AGAGTGC555.182301E-922.010
GTAGCTG353.1964384E-522.08
GCATTCC250.002572271722.04
GTTCGGC353.1964384E-522.08
CTGCCTA250.002572271722.01
GTCGTGC504.5709385E-822.07
Produced by FastQC (version 0.11.5)