FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n01_WTA002-Lib2-C8T.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n01_WTA002-Lib2-C8T.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122620
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGG353.185076E-522.08
AGACTGG250.002567357622.010
ATGGGAC250.002567357622.010
GGTCGAG250.002567357622.07
TCACATC302.852442E-422.03
ACACATG250.002567357622.07
TCGGGGT353.185076E-522.03
AAGACAC353.185076E-522.04
GCACGAG353.185076E-522.03
TTCCGGT302.852442E-422.01
CGATTGT302.852442E-422.03
CTAAGAC353.185076E-522.02
CCTAAGA353.185076E-522.01
TAGTTCC250.002567357622.04
AAAGTGA250.002567357622.01
CTGCGGC250.002567357622.09
CGGGGTC302.852442E-422.04
GCTTCAC250.002567357622.01
AGTGAGT250.002567357622.02
GGGGTCC302.852442E-422.05
Produced by FastQC (version 0.11.5)