FastQCFastQC Report
Wed 22 Jul 2020
000000000-G59K8_l01_n01_WTA002-Lib1-C8A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G59K8_l01_n01_WTA002-Lib1-C8A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences348407
Sequences flagged as poor quality0
Sequence length28
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGA250.00257678722.09
CACGGAA250.00257678722.08
TTGTCCG403.603327E-622.05
AGGCGTA353.2068907E-522.04
AGGCGAA403.603327E-622.08
AACGGGA302.8670992E-422.01
GGGCGTA353.2068907E-522.02
CAGGCGT403.603327E-622.03
ACGTGGG250.00257678722.06
CCGAGGT403.603327E-622.010
CGTACTG250.00257678722.07
TCCCGAG454.0645864E-722.08
TTCGGGC250.00257678722.010
GTCCACC353.2068907E-522.07
TGGGAGA302.8670992E-422.01
GCGCTGG302.8670992E-422.02
CCACGGT403.603327E-622.03
AGGGAAG707.2759576E-1222.03
GCGTAGG403.603327E-622.04
TTGGGCG454.0645864E-722.01