FastQCFastQC Report
Thu 18 Jul 2019
000000000-G4545_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G4545_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310723
Sequences flagged as poor quality0
Sequence length162
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG434951.3137613747812789No Hit
TACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCTCCTGCTTT197520.596606843882741No Hit
TACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCTCCTGCTTT176340.5326329022391786No Hit
GTACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCTCCTGCTT164940.49819933591544807No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCCTCCTGCTTT161140.48672148047420455No Hit
GTACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCTCCTGCTT156630.47309907835841297No Hit
TACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCTCCTGCTTT150810.45551983660366635No Hit
ACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCTCCTGCTTTA143760.4342253942718856No Hit
GTACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCCTCCTGCTT130290.3935394172209514No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTCCTCCTGCTTT122490.36997960868366214No Hit
TACGCCGTCCAGCTGAACCTTCCACCGGGAGCTGCATGTCCTCCTGCTTT122470.3699191989181819No Hit
CGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCTCCTGCTTTAT120190.3630324856534358No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCTCCTGCTTTA118110.356749870043492No Hit
GTACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCTCCTGCTT113070.34152660914247435No Hit
TACGCCGTCCAGCTGAATTCCGGTCCGGGAGCTGCATGTCCTCCTGCTTT106800.3225881476644225No Hit
CGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCTCCTGCTTTAT102350.30914697484507164No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCTCCTGCTTTA99680.30108227115346103No Hit
GTACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTCCTCCTGCTT99450.30038755885043844No Hit
GTACGCCGTCCAGCTGAACCTTCCACCGGGAGCTGCATGTCCTCCTGCTT98630.29791075846574905No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCCTCCTGCTTTA95460.28833581063713276No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCCTCCTGCTTTT94460.2853153223631213No Hit
TACGCCGTCCAGCTGACGTTACTCCCGGGAGCTGCATGTCCTCCTGCTTT92570.27960659952523964No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCTCCTGCTTTT92460.2792743458150984No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAACGTACTTTTT86590.26154407964665116No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCTCCTGCTTTAT86300.26066813804718786No Hit
GTACGCCGTCCAGCTGAATTCCGGTCCGGGAGCTGCATGTCCTCCTGCTT85730.2589464597310014No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTCCTCCTGCTTTT85080.25698314235289393No Hit
ACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCTCCTGCTTTT85020.2568019130564532No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCCTCCTGCTTTTT82970.25060991209472977No Hit
CGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCTCCTGCTTTTT82460.24906946307498395No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCCTCCTGCTTTAT81370.24577713085631145No Hit
GTACGCCGTCCAGCTGACGTTACTCCCGGGAGCTGCATGTCCTCCTGCTT81050.24481057460862776No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAACGTACTTTTTT79900.24133701309351463No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAACGTACTTTT77180.2331212849882035No Hit
ACGCCGTCCAGCTGAACCTTCCACCGGGAGCTGCATGTCCTCCTGCTTTA75290.22741256215032182No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCTCCTGCTTTT74570.22523781059303358No Hit
ACGCCGTCCAGCTGAATTCCGGTCCGGGAGCTGCATGTCCTCCTGCTTTA74120.22387859086972847No Hit
ACGCCGTCCAGCTGAACCTTCCACCGGGAGCTGCATGTCCTCCTGCTTTT71210.21508896999235513No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTCCTCCTGCTTTTT70940.21427343815837208No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCATTAACGTTTT67890.20506094892263713No Hit
CGCCGTCCAGCTGAATTCCGGTCCGGGAGCTGCATGTCCTCCTGCTTTAT67860.2049703342744168No Hit
CGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCTCCTGCTTTTT66370.20046980674613973No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCTCCTGCTTTTT65740.19856689913351255No Hit
CGCCGTCCAGCTGAACCTTCCACCGGGAGCTGCATGTCCTCCTGCTTTAT64600.19512354250113947No Hit
CGCCGTCCAGCTGAACCTTCCACCGGGAGCTGCATGTCCTCCTGCTTTTT64340.1943382155498965No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGTCAGTTTTT64220.19397575695701513No Hit
ACGCCGTCCAGCTGACGTTACTCCCGGGAGCTGCATGTCCTCCTGCTTTA63120.19065321985560255No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTCCTCCTGCTTTA60470.1826489259294722No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60440.18255831128125186No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCGCCTCGGTTTT60240.18195421362644956No Hit
GTACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAACGTACTTT56720.17132209490192926No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGTCAGTTTTTT56260.16993267029588402No Hit
TACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCATTAACGTTT55090.1663986990152906No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTCTTAGAGTTTT54820.16558316718130753No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGACTGAGTTTT54740.1653415281193866No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTTAATTATGTTTT54360.16419374257526226No Hit
CGCCGTCCAGCTGACGTTACTCCCGGGAGCTGCATGTCCTCCTGCTTTAT54260.16389169374786114No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCATTAACGTTTTT54250.163861488865121No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGACTGAGTTTTT53070.1602973127017875No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGTCAGTTTT51250.15480002404308665No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCGCCTCGGTTT50570.15274609201675887No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCGGATTGTTTT50160.1515076918244142No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTCTTAGAGTTTTT50150.15147748694167407No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGACGAGTTTT49930.15081297952139155No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCATCAAGCTTTT49690.1500880623356288No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTCCTCCTGCTTTAT49580.14975580862548754No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTGCGCAATATTTT48740.14721859847531793No Hit
CGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCGGATTGTTTTT48250.14573855922105233No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCGCCTCGGTTTTT47710.14410749555308613No Hit
ACGCCGTCCAGCTGAATTCCGGTCCGGGAGCTGCATGTCCTCCTGCTTTT46580.1406943438034532No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGACGAGTTTTT46290.1398184022039899No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGACTGAGTTT45810.13836856783246437No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTTAATTATGTTT45590.13770406041218186No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTGCGCAATATTTTT45180.1364656602198372No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCTCTACTATTTT45150.13637504557161684No Hit
ACGCCGTCCAGCTGACGTTACTCCCGGGAGCTGCATGTCCTCCTGCTTTT44920.13568033326859422No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTGCAACGCCTTTT44440.1342304988970687No Hit
CGCCGTCCAGCTGAATTCCGGTCCGGGAGCTGCATGTCCTCCTGCTTTTT43140.13030386414085382No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTTAATTATGTTTTT42550.12852177605918708No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCATCAAGCTTT42410.12809890770082547No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCAACGCCTTTT42400.12806870281808536No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCTCTACTATTTTT42340.12788747352164467No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTCTTAGAGTTT42170.12737399051506273No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCATCAAGCTTTTT42170.12737399051506273No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGGTCTATTTTT42060.12704173680492145No Hit
TACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCGGATTGTTT41480.12528985360599482No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTATGCTCAGTTTTT41470.1252596487232547No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTATAACTCTTTT41360.12492739501311345No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGGTCTATTTTTT41260.1246253461857123No Hit
ACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTATGCTCAGTTTT41250.1245951413029722No Hit
GTACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCGCCTCGGTT40670.12284325810404556No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTGCGCAATATTT40500.12232977509746361No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCATGGTTTTTT40500.12232977509746361No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCTCAATTGTTTT40380.12196731650458224No Hit
GTACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGTCAGTTT40350.12187670185636189No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCGATTGCTTTT39730.1200039991264748No Hit
GTACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCATTAACGTT39690.11988317959551434No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTAACGTACTTTTT39500.11930928682345215No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGACGAGTTT39260.11858436963768941No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCGGTCAGTTTT39140.11822191104480803No Hit
GTACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGACTGAGTT38750.11704392061794358No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCTCTACTATTT38700.116892896204243No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCAACGCCTTTTT38540.11640961808040116No Hit
CGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCTCAATTGTTTTT38370.11589613507381923No Hit
CGCCGTCCAGCTGACGTTACTCCCGGGAGCTGCATGTCCTCCTGCTTTTT38350.11583572530833901No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCGATTGCTTTTT38320.11574511066011865No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCATGGTTTTTTT38290.1156544960118983No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTGCAACGCCTTTTT38110.11511080812257625No Hit
ACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCATTAACGTTTT37910.11450671046777396No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTGTCTGGAGTTTT36360.10982495364305622No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTAGGTCTATTTT36350.10979474876031609No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCGCCTTCTTTTT36060.10891880716085278No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTAACGTACTTTTTT35960.10861675833345164No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTGCAACGCCTTT35770.10804286556138946No Hit
CGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTATAACTCTTTTT35710.10786163626494878No Hit
TACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCAACGCCTTT35210.10635139212794305No Hit
TACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCATGGTTTTT35120.10607954818328202No Hit
ACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTTCATGCTGTTTT35050.1058681140041012No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTTGATTCCGTTTT35000.10571708959040065No Hit
TACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTCTCAATTGTTT34960.10559627005944018No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCGAGATGTTTT34770.10502237728737801No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTAGCCGTACTTTT34610.1045390991635362No Hit
ACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTTGGTCAGTTTTT34390.10387459174325366No Hit
CGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCGGTCAGTTTTT34280.1035423380331124No Hit
GTACGCCGTCCAGCTGAGGAGTCTGCCGGGAGCTGCATGTTAATTATGTT34170.10321008432297116No Hit
GTACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTGGACGAGTT34060.10287783061282989No Hit
ACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTGATGCGTTTTTT34010.10272680619912931No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTATAACTCTTT33790.10206229877884679No Hit
GTACGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTCCGGATTGTT33770.10200188901336657No Hit
TACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTAACGTACTTTT33710.10182065971692587No Hit
TACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTATGCTCAGTTT33660.1016696353032253No Hit
GTACGCCGTCCAGCTGAACGCTTAACCGGGAGCTGCATGTTCTTAGAGTT33640.10160922553774508No Hit
TACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTGCGATTGCTTT33460.10106553764842302No Hit
ACGCCGTCCAGCTGATTCTGGTTCCGGGAGCTGCATGTCCAATTCCTTTT33450.10103533276568291No Hit
ACGCCGTCCAGCTGAGTTATATGCCGGGAGCTGCATGTCCGAGATGTTTT33420.10094471811746256No Hit
CGCCGTCCAGCTGACTAGATCTCCGGGAGCTGCATGTGTCTGGAGTTTTT33320.10064266929006142No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAC159.1934184E-5156.000023
TCTTAAG100.005698897156.02
GTACTAC100.005698897156.01
TATAGTC100.005698897156.03
AAGTGCC100.005698897156.03
CTAATCC100.005698897156.03
GTACGCC584850.0154.933041
AGCGCCG16100.0150.186347
ACTGCGC1050.0148.571435
CAGCGCC4050.0148.29636
TTACCGC750.0145.599995
AAGCGCC4200.0144.857136
TTCGCCG15450.0144.388357
TCGCCGT46550.0144.270688
TAGCGCC3650.0143.178076
AACGCCG11800.0141.457637
CTCGCCG9800.0140.877557
AGGCGCC4050.0140.592596
CTTACGC950.0139.578955
AATCGCC2800.0139.28576
Produced by FastQC (version 0.11.5)