FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377300
Sequences flagged as poor quality0
Sequence length56
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATACTCCTTTCAAGACCTAGCTAGCCCATTAGAATTCAAAAAAAGCACCGACTC9630.25523456135701034No Hit
CGATACTCCTTTCAAGACCTAGCTAGCCGACCCGAATTCAAAAAAAGCACCGACTC8950.2372117678240127No Hit
CGATACTCCTTTCAAGACCTAGCTAGCGCCGCCGAATTCAAAAAAAGCACCGACTC8020.21256294725682479No Hit
ATACTCCTTTCAAGACCTAGCTAGCCCACAAGAATTCAAAAAAAGCACCGACTCGG3840.10177577524516301No Hit
GATACTCCTTTCAAGACCTAGCTAGCACACGGGAATTCAAAAAAAGCACCGACTCG3810.10098065200106017No Hit
ATACTCCTTTCAAGACCTAGCTAGCTTGGACGAATTCAAAAAAAGCACCGACTCGG3790.10045056983832494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGGT550.050.021
AGGAGAC550.050.019
TAGACCC204.1802932E-450.032
CGGTTGC252.0847861E-550.026
AACCAGG204.1802932E-450.013
GTCTCCG204.1802932E-450.024
TCAGGTC355.2767064E-850.030
ACGGGCC507.2759576E-1250.020
GCCGAAT507.2759576E-1250.031
TCTACAC550.050.022
GTTGTCC204.1802932E-450.023
CTACGTA204.1802932E-450.019
GTGTCTA550.050.019
CTCGCTC1400.050.03
AGACAAT507.2759576E-1250.022
CGTGTCG204.1802932E-450.017
CTACACT550.050.023
ACGTATC700.050.028
CAGTCTA204.1802932E-450.015
TGTACGG252.0847861E-550.022
Produced by FastQC (version 0.11.5)