FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_cDNA2_D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_cDNA2_D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347131
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.13078636019254977No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTCG250.001254558440.032
TCAACGC2000.032.512
CAACGCA1950.032.05128513
ATCAACG1650.031.8181811
AACGCAG2200.029.54545614
GCTCTTA1151.8189894E-1228.260876
GCATAGC1250.028.01
CGCAGAG2450.027.55102216
AGAGTAC2400.027.08333219
GGTATCA1950.026.9230798
AAGCAGT2450.026.5306131
AGCAGTG2550.026.4705892
TATCAAC2000.026.2510
GTGGTAT2100.026.1904756
ACGCAGA2300.026.08695615
TGGTATC2050.025.6097567
CATAGCT1301.2732926E-1124.9999982
GTATCAA2100.024.9999989
GAGTACT2800.023.21428520
TAGCTCT1403.274181E-1123.2142854
Produced by FastQC (version 0.11.5)