FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_cDNA2_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_cDNA2_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306576
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3800.12394968947340952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC2400.032.29166412
ATCAACG2250.032.2222211
GTATCAA2350.031.9148949
CAACGCA2550.030.39215713
AAGCAGT2450.029.5918371
CGCAGTC350.006525720528.57142824
TATCAAC2650.027.35849210
AGAGTAC2750.027.27272619
ACGCAGA2850.027.19298415
AACGCAG2900.026.72413814
GGTATCA2750.026.3636368
CGCAGAG2950.026.27118716
TGGTATC2850.025.4385977
GAGTACT3050.024.59016620
AGCAGTG2850.024.5614032
GTACTTT3000.024.16666622
AGTGGTA3100.023.3870965
GCAGAGT3300.022.72727417
CAGAGTA3450.022.46376818
AGTACTT3250.022.30769221
Produced by FastQC (version 0.11.5)