FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_cDNA2_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_cDNA2_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297801
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.12357245274528963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG1800.034.72222511
TCAACGC2100.032.14285712
CAACGCA2250.030.013
AACGCAG2350.028.72340214
GGTATCA2250.027.7777798
GTATCAA2200.027.2727289
ACGCAGA2500.027.015
AGTGGTA2500.027.05
AAGCAGT2500.027.01
AGAGTAC2850.026.3157919
TGGTATC2400.026.0416667
GAGTACT2700.025.92592420
CGCAGAG2800.025.89285716
GTGGTAT2450.025.5102046
CAGTGGT2750.025.4545444
TATCAAC2500.025.010
CAGAGTA3000.024.16666818
AGTACTT3000.024.16666821
GTACTTT2950.023.72881522
AGCAGTG3000.023.3333342
Produced by FastQC (version 0.11.5)