FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_cDNA2_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_cDNA2_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437774
Sequences flagged as poor quality0
Sequence length56
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5740.1311178827431506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGCA2450.032.6530613
ATCAACG2300.032.60869611
TCAACGC2550.031.3725512
GCATAGC1050.030.9523811
AACGCAG2650.030.18867714
ATAGCTC1350.027.7777773
GTATCAA2700.027.7777779
AGAGTAC2950.027.11864319
CGCAGAG3150.026.98412716
GAGTACT3100.026.61290420
TATCAAC2850.026.3157910
ACGCAGA3000.025.83333215
GGTATCA2850.025.4385978
GTACTTT3150.025.39682622
AGTGGTA2900.025.05
GCTCTTA1401.8189894E-1225.06
CAGAGTA3250.024.61538518
ATATAAG951.9981053E-723.68421240
GCAGAGT3450.023.18840417
TGGTATC3050.022.950827
Produced by FastQC (version 0.11.5)