FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_GDO2_F.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_GDO2_F.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444684
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAGAG1700.050.00000427
CCCTCAC4250.050.00000430
GGGTCCA6050.050.00000422
GGTCCAC6050.050.00000423
CAGGGTG1700.050.00000429
TGACAGA1850.050.00000416
GGTGCCC1700.050.00000432
TTCATTT1700.050.00000417
CAGAGGA1850.050.00000419
ACGCGAC150.00842761550.049
TCCGGAA150.00842761550.050
GTGTTTA1200.050.029
CTTGTGA204.1811046E-450.015
TCTGCCT4350.050.017
GTTGAGC8150.050.025
TTGATGA150.00842761550.050
GCCCCGG252.085393E-550.050
ATTTACT150.00842761550.049
TTCTATC204.1811046E-450.029
CTTAAAT150.00842761550.049
Produced by FastQC (version 0.11.5)