FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_GDO2_D.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_GDO2_D.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397135
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTTA1250.050.00000429
GTCATCG1250.050.00000417
TGTTTAC1250.050.00000430
GTCTATT2650.050.00000417
CCGTCAT1250.050.00000415
CTACGTC2650.050.00000428
GCTAATG6650.050.00000419
CCTGCTA6550.050.00000416
TGAGCCT6550.050.00000427
TACGTCC2650.050.00000429
CGGACAC1150.050.030
GTGTTGT1550.050.021
TTGATGC18200.050.029
ATTTAAG204.1805604E-450.040
TGTCACG2050.050.017
TGTCAAG204.1805604E-450.022
CTATGGA950.050.020
CGTGGCT204.1805604E-450.029
GGGGAGG1000.050.024
GCCTTTT850.050.021
Produced by FastQC (version 0.11.5)