FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_GDO2_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_GDO2_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711285
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTGT2500.050.00000421
GTGGGGC2300.050.00000421
GACGTCC1150.050.00000423
TGTTGTC2500.050.00000422
CCCGTGG1150.050.00000415
CCGTAGT2300.050.00000416
GTGCTCA15750.050.00000421
GTCCGGA1150.050.00000426
TGGGGCT2300.050.00000422
TTTGCTT750.050.00000420
GGTTGCC5450.050.00000431
TTGCAGG2500.050.00000418
TCCTTGG1500.050.00000419
CGTCCGG1150.050.00000425
GGAGGGC1150.050.00000430
GCATAGT750.050.0000041
CGGACAA355.2856194E-850.050
CTTGTGA402.6811904E-950.015
CCTTCCG204.1828092E-450.050
CCTTCAT2600.050.015
Produced by FastQC (version 0.11.5)