FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_GDO2_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_GDO2_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583272
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTG3950.050.00000419
GAACACC3650.050.00000431
CCTTGTC3950.050.00000415
TGATGAC5250.050.00000423
CCTCTGC5250.050.00000415
AGGTCAC150.00842892450.049
CCACAAG2500.050.015
ACGCGAG301.0469848E-650.049
CGGACAC2400.050.030
CCCTGCC204.1821855E-450.050
AGCACAA204.1821855E-450.049
CCTTCGC150.00842892450.050
GTGTTTG150.00842892450.050
GTGTTTA2300.050.029
CTGCTTG150.00842892450.050
GCCCCTC204.1821855E-450.050
CCTTCAT2300.050.015
CTGCTAA10250.050.017
GTCATCG2300.050.017
ACAACAG204.1821855E-450.049
Produced by FastQC (version 0.11.5)