FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n02_GDO2_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n02_GDO2_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences813300
Sequences flagged as poor quality0
Sequence length56
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTG5950.050.00000417
GGCAGAG3300.050.00000427
GGGCTGT3300.050.00000424
CGGTTGC5900.050.00000426
TTCGTGT5900.050.00000418
CGGGCCG5250.050.00000421
GTACTGT1650.050.00000429
GTCCGGA1250.050.00000426
TGGGGCT3300.050.00000422
GGACGGC3300.050.00000423
GTTCACA2500.050.00000429
CAGTACT1650.050.00000427
GTGACGT1250.050.00000421
TGTACGG5900.050.00000422
GGGGCTG3300.050.00000423
GTGTTTA3050.050.029
GTTGAGG3050.050.023
CTTAAGC252.0869342E-550.09
CAGGTAT1450.050.016
ACAAATT252.0869342E-550.049
Produced by FastQC (version 0.11.5)