FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377300
Sequences flagged as poor quality0
Sequence length26
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG14980.3970315398886827No Hit
ACGATCGATAGGTAAGGTCTTGTGGA12730.33739729658097006No Hit
GATCGATAACGCATTTCTTGTGGAAA10030.2658362046117148No Hit
ATACTGTATCTCTTGTGGAAAGGACG9860.26133050622846543No Hit
ATCGATTCCTTGGTTCTTGTGGAAAG9760.2586800954147893No Hit
ATACACGATCTCTTGTGGAAAGGACG9620.25496952027564274No Hit
TCGATCGTTACCATCTTGTGGAAAGG9620.25496952027564274No Hit
CGATCATGATCGTCTTGTGGAAAGGA9610.2547044791942751No Hit
CGATCGATACAGGTATTCTTGTGGAA9340.24754836999734958No Hit
GATCGCGCGGTTCTTGTGGAAAGGAC8950.2372117678240127No Hit
TAAGTAGAGTCTTGTGGAAAGGACGA8780.2327060694407633No Hit
GATAGGTCGCATCTTGTGGAAAGGAC7380.19560031804929764No Hit
TAACAATGGTCTTGTGGAAAGGACGA6700.17757752451630002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGG1450.020.0000026
CTTAACT250.004477995420.01
TTCTACA356.685361E-520.01
GAAATAC305.4572197E-420.011
AGACTAG250.004477995420.08
TCGTTAC1050.020.05
GATACAG1000.020.06
GATCGTC950.020.08
TACTTCT250.004477995420.09
GATCGCG950.020.01
GTCTGAG305.4572197E-420.03
AGATAAG250.004477995420.010
TGGACCC250.004477995420.010
CGCATTT1200.020.010
AACTTCA250.004477995420.03
GCAAACT250.004477995420.01
TCGGGAC408.2271545E-620.01
GATCATG900.020.02
TAAGTCC250.004477995420.06
CGATTGA250.004477995420.01
Produced by FastQC (version 0.11.5)