FastQCFastQC Report
Fri 5 Apr 2019
000000000-G3JC4_l01_n01_cDNA2_C.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G3JC4_l01_n01_cDNA2_C.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306576
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA800.020.0000021
GAGCGTA408.2188835E-620.0000027
CGACTGT408.2188835E-620.0000023
ATCGAGT408.2188835E-620.0000021
ATGGGAG551.5632395E-820.01
AGTCCAA250.00447611420.04
ACCATTC356.68013E-520.06
CGCGGAT356.68013E-520.09
ACTGACA250.00447611420.03
ACGCACG451.0151216E-620.05
GATCCGA250.00447611420.09
GATCCCG250.00447611420.09
ACACATC356.68013E-520.07
ACCGAAA250.00447611420.09
TTGTAAA250.00447611420.06
CACGAGG250.00447611420.08
CGCGAAA703.092282E-1120.010
CCGCTGT305.454012E-420.09
CTTGGAT356.68013E-520.010
GCCGAAG305.454012E-420.03
Produced by FastQC (version 0.11.5)