FastQCFastQC Report
Thu 19 Sep 2019
000000000-G34FW_l01_n01_Cas13PlasLib.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-G34FW_l01_n01_Cas13PlasLib.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020525
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT87958221.8772921446826No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT75779118.848060887570654No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG68349817.000217633269287No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG64393816.01626653235585No Hit
TAAGTAGAGTCTTGTNGAAAGGACGAAACACCGAACCCCTACCAACTGGT2085875.188053798944168No Hit
CGATCATGATCGTCTNGTGGAAAGGACGAAACACCGAACCCCTACCAACT1715994.268074443014283No Hit
ATACACGATCTCTTGNGGAAAGGACGAAACACCGAACCCCTACCAACTGG1588413.950752700207062No Hit
GATCGCGCGGTTCTTNTGGAAAGGACGAAACACCGAACCCCTACCAACTG1493443.7145397678163925No Hit
CGATCATGATCGTCTTGTGGAAAGGACGCAACACCGAACCCCTACCAACT70950.17646949092469266No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTGG58260.14490644878467365No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAAAACCGAACCCCTACCAACTGGT57080.14197150869600364No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACT49700.12361569695499967No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGACACACCGAACCCCTACCAACTG46130.11473625956809122No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACTG44190.10991101908332866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGCG858150.0142.534261
TACACGA920050.0142.491172
ATACACG921200.0142.398481
TCGCGCG858950.0142.326753
TGATCGT1018250.0142.252097
GTAGAGT1150350.0142.235374
GATCATG1018050.0142.230972
GATCGTC1018250.0142.224068
ATCATGA1019550.0142.070713
TAGAGTC1151250.0142.062215
AAGTAGA1152650.0142.038212
AGTAGAG1151950.0142.03783
ATCGTCT1020500.0142.036339
GCGGTTC860300.0142.020487
ATCGCGC861800.0142.013382
CGATCAT1020100.0142.00811
AGTCTTG1153900.0142.0088
CGCGCGG859950.0141.945514
ATGATCG1019700.0141.867896
TCTCTTG929600.0141.710429
Produced by FastQC (version 0.11.5)