FastQCFastQC Report
Fri 14 Feb 2025
000000000-DT6M2_l01_n02_HS_Penseq_Testrun_A01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DT6M2_l01_n02_HS_Penseq_Testrun_A01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3822
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATACCGCCGCCACCCTAACGCCTCGAAGAACTAATGGCAGCCCAC50.13082155939298795No Hit
CTCCTAGGCCGTTTGGGTGGCGGTATAGTCGTCTTGCGCACGAAATACCG50.13082155939298795No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGCCTCGATGAGTAGGAGGG40.10465724751439037No Hit
CGCCTAGGCTGTCCCGAAGGTATCTCGCGCTTGTACGGCTTTGGCTCGGA40.10465724751439037No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC40.10465724751439037No Hit
CCCTAACGTTGCCGTCAACCGCCACGTCCCGGTTCCGGAATTTTAACCGG40.10465724751439037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)