FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC222191.02459016393442No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCC451.8442622950819672No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGCGGGATTCACCACTTTTCCC190.778688524590164No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATAGATCGGAAGAGCGTCGTGTAG120.49180327868852464Illumina Single End PCR Primer 1 (100% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC100.4098360655737705No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCC70.28688524590163933No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGTGATTCACCACTTTTCCC60.24590163934426232No Hit
TTTGAGGAGCGAGAGGCAGAGACACGATAGATCGGAAGAGCGTCGTGTAG50.20491803278688525Illumina Single End PCR Primer 1 (100% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCA40.16393442622950818No Hit
TTTGAGGAGCGATAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC30.12295081967213116No Hit
TTTGAGGAGCGAGAGGCAGAGACACGATCGATCGGAAGAGCGTCGTGTAG30.12295081967213116Illumina Single End PCR Primer 1 (95% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGAGGGATTCACCACTTTTCCC30.12295081967213116No Hit
TTTTAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC30.12295081967213116No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACC30.12295081967213116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)