FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA435194.77238074493575No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCCA150.326726203441516No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACCA90.1960357220649096No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCCA80.17425397516880856No Hit
TTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA80.17425397516880856No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCCA70.15247222827270746No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCCA70.15247222827270746No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCAA70.15247222827270746No Hit
TTGAGGAGCGAGAGGAAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA60.1306904813766064No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCACA60.1306904813766064No Hit
TTGAGGAGCTAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCCA50.10890873448050534No Hit
TTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCCCTTTTCCCA50.10890873448050534No Hit
TTGAGGAGCGAGAAGCAGAGACACGATCAGATCGGAAGAGCGTCGTGTAG50.10890873448050534Illumina Single End PCR Primer 1 (100% over 22bp)
TTGAGGAGCGAGAGGCAGTTATTTTTTGGTGGGATTCACCACTTTTCCCA50.10890873448050534No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)