FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC428294.98669032830523No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC210.4658385093167702No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCC110.2440106477373558No Hit
TTTGAGGAGCGAGAAGCAGAGACACGAGATCGGAAGAGCGTCGTGTAGGG100.22182786157941436Illumina Single End PCR Primer 1 (100% over 24bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCC90.19964507542147292No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCAC80.1774622892635315No Hit
TTTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC70.15527950310559005No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCC60.13309671694764863No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACC60.13309671694764863No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)