FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC195795.09232264334305No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATCAGATCGGAAGAGCGTCGTGTA100.4859086491739553Illumina Single End PCR Primer 1 (100% over 21bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC70.3401360544217687No Hit
TTTGAGGAGCGATAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC50.24295432458697766No Hit
TTTGAGGAGCGAGAGGCAGAGACACGATCAGATCGGAAGAGCGTCGTGTA50.24295432458697766Illumina Single End PCR Primer 1 (100% over 21bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTTGGTGGGATTCACCACTTTTCCC40.1943634596695821No Hit
TTTGAGGAGCTAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC40.1943634596695821No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCAC40.1943634596695821No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACC40.1943634596695821No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCC30.1457725947521866No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCC30.1457725947521866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)