FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC368994.83290488431876No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATAGATCGGAAGAGCGTCGTGTAG180.46272493573264784Illumina Single End PCR Primer 1 (100% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC150.3856041131105398No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACC90.23136246786632392No Hit
TTTGAGGAGCGAGAGGCAGAGACACGATAGATCGGAAGAGCGTCGTGTAG80.2056555269922879Illumina Single End PCR Primer 1 (100% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCC80.2056555269922879No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCA60.15424164524421594No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTTGGTGGGATTCACCACTTTTCCC50.12853470437017994No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGTGTGGGATTCACCACTTTTCCC50.12853470437017994No Hit
TTTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC40.10282776349614395No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATCGATCGGAAGAGCGTCGTGTAG40.10282776349614395Illumina Single End PCR Primer 1 (95% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCAC40.10282776349614395No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCC40.10282776349614395No Hit
TTTTAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC40.10282776349614395No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTTGGATTCACCACTTTTCCC40.10282776349614395No Hit
TTTGAGGAGCGAGAGGAAGTTATTTTTGGGTGGGATTCACCACTTTTCCC40.10282776349614395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)