FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6494
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC614494.61040960886973No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC200.3079765937788728No Hit
TTTGAGGAGCGAGAGGCAGAGACACGATCAGATCGGAAGAGCGTCGTGTA190.29257776408992914Illumina Single End PCR Primer 1 (100% over 21bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTTGGTGGGATTCACCACTTTTCCC160.24638127502309826No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATCAGATCGGAAGAGCGTCGTGTA130.20018478595626735Illumina Single End PCR Primer 1 (100% over 21bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCC120.1847859562673237No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACC100.1539882968894364No Hit
TTTGAGGAGCGATAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC90.13858946720049276No Hit
TTTTAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC90.13858946720049276No Hit
TTTGAGGAGCTAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC80.12319063751154913No Hit
TTTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC80.12319063751154913No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCC80.12319063751154913No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)