FastQCFastQC Report
Mon 24 Mar 2025
000000000-DRK9T_l01_n02_LL15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-DRK9T_l01_n02_LL15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4686
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC433692.53094323516858No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGGGGGATTCACCACTTTTCCC851.8139137857447716No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGCGGGATTCACCACTTTTCCC260.5548442168160478No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATAGATCGGAAGAGCGTCGTGTAG160.34144259496372176Illumina Single End PCR Primer 1 (100% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTAACCACTTTTCCC120.2560819462227913No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCA90.19206145966709345No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTACC90.19206145966709345No Hit
TTTGAGGAGCGAGAGGCAGAGACACGATAGATCGGAAGAGCGTCGTGTAG80.17072129748186088Illumina Single End PCR Primer 1 (100% over 22bp)
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGTATTCACCACTTTTCCC80.17072129748186088No Hit
TTTGAGTAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC70.14938113529662825No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACAACTTTTCCC70.14938113529662825No Hit
TTTGAGGAGCGATAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCCC60.12804097311139565No Hit
TTTGAGGAGCGAGAGGCAGTTATTTTTGGGTGGGATTCACCACTTTTCAC50.10670081092616304No Hit
TTTGAGGAGCGAGAAGCAGAGACACGATAGCTCGGAAGAGCGTCGTGTAG50.10670081092616304Illumina Single End PCR Primer 1 (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)